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| Status |
Public on May 22, 2011 |
| Title |
Sensing prokaryotic mRNA signifies microbial viability and promotes immunity |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
MyD88-independent signal transduction associated with Toll-like receptors (TLRs) 3 and TLR4 is mediated through the adapter protein TRIF (TIR-domain-containing adapter-inducing interferon-beta). It has been proposed that TLR signalling is important for the transcription of crucial inflammasome components like NLRP3, a process that has been termed "priming". In order to test whether TRIF signalling was required for the priming of inflammasome components, we performed a genome wide transcriptional analysis on wild-type and Trif-knockout bone marrow derived macrophages (BMMs) before and 1, 3 and 6 hours after phagocytosis of E. coli. These results indicated that TRIF was involved in the activation and not transcriptional priming of the NLRP3 inflammasome.
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| |
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| Overall design |
Bone marrow derived macrophages from WT and Trif knockout mice, stimulated with E.coli for up to 6hrs.
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| Contributor(s) |
Sander LE, Davis MJ, Boekschoten MV, Amsen D, Dasher CC, Ryffel B, Swanson JA, Müller M, Blander JM |
| Citation(s) |
21602824, 27348412 |
| Submission date |
Mar 15, 2011 |
| Last update date |
Mar 30, 2020 |
| Contact name |
Guido Hooiveld |
| E-mail(s) |
guido.hooiveld@wur.nl
|
| Organization name |
Wageningen University
|
| Department |
Div. Human Nutrition & Health
|
| Lab |
Nutrition, Metabolism & Genomics Group
|
| Street address |
HELIX, Stippeneng 4
|
| City |
Wageningen |
| ZIP/Postal code |
NL-6708WE |
| Country |
Netherlands |
| |
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| Platforms (1) |
| GPL11533 |
[MoGene-1_1-st] Affymetrix Mouse Gene 1.1 ST Array [transcript (gene) version] |
|
| Samples (24)
|
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| Relations |
| BioProject |
PRJNA137747 |
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