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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 21, 2011 |
| Title |
Inflammation switches the differentiation program of Ly6Chi monocytes from anti-inflammatory macrophages to inflammatory dendritic cells in the colon |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Dendritic cells (DCs) and macrophages (MPs) are important for immunological homeostasis in the colon. We found that F4/80hi CX3CR1hi (CD11b+CD103-) cells account for 80% of mouse colonic lamina propria (cLP) MHC-IIhi cells. Both CD11c+ and CD11c- cells within this population were identified as MPs based on multiple criteria, including a MP transcriptome revealed by microarray analysis. These MPs constitutively released high levels of IL-10 at least partially in response to the microbiota via an MyD88-independent mechanism. In contrast, cells expressing low to intermediate levels of F4/80 and CX3CR1 were identified as DCs, based on phenotypic and functional analysis and comprise three separate CD11chi cell populations: CD103+CX3CR1-CD11b- DCs, CD103+CX3CR1-CD11b+ DCs and CD103-CX3CR1intCD11b+ DCs. In non-inflammatory conditions, Ly6Chi monocytes differentiated primarily into CD11c+, but not CD11c- MPs. In contrast, during colitis, Ly6Chi monocytes massively invaded the colon and differentiated into pro-inflammatory CD103-CX3CR1intCD11b+ DCs, which produced high levels of IL-12, IL-23, iNOS and TNF. These findings demonstrate the dual capacity of Ly6Chi blood monocytes to differentiate into either regulatory MPs or inflammatory DCs in the colon, and that the balance of these immunologically antagonistic cell types is dictated by microenvironmental conditions.
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| Overall design |
FACS sorted expression from normal controls
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| Contributor(s) |
Rivollier A, He J, Kole A, Valatas V, Kelsall BL |
| Citation(s) |
22231304 |
| Submission date |
Mar 09, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Dan Sturdevant |
| E-mail(s) |
dsturdevant@niaid.nih.gov
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| Phone |
4063639248
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| Organization name |
NIH
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| Department |
NIAID
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| Lab |
RTS
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| Street address |
903 S 4th street
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| City |
Hamilton |
| State/province |
MT |
| ZIP/Postal code |
59840 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (10)
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| GSM687732 |
normal mouse macrophage sorted CD11c-F4/80hi rep 1 |
| GSM687733 |
normal mouse macrophage sorted CD11c-F4/80hi rep 2 |
| GSM687734 |
normal mouse dendritic sorted CD11chi-F4/80- rep 1 |
| GSM687735 |
normal mouse dendritic sorted CD11chi-F4/80- rep 2 |
| GSM687736 |
normal mouse dendritic sorted CD11chi-F4/80- rep 3 |
| GSM687737 |
normal mouse macrophage sorted CD11cint F4/80hi rep 1 |
| GSM687738 |
normal mouse macrophage sorted CD11cint F4/80hi rep 2 |
| GSM687739 |
normal mouse macrophage sorted CD11cint F4/80hi rep 3 |
| GSM687740 |
normal mouse dendritic sorted CD11cint F4/80int rep 1 |
| GSM687741 |
normal mouse dendritic sorted CD11cint F4/80int rep 2 |
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| Relations |
| BioProject |
PRJNA137881 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE27859_RAW.tar |
44.7 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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