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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Oct 05, 2011 |
| Title |
Identification of a Potently Oncogenic CALM-AF10 Minimal-Fusion Mutant (mRNA) |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
The t(10;11) p (13;q14) translocation, giving rise to CALM-AF10, is a recurring chromosomal translocation observed in several types of acute leukemias as well as in lymphoma. We have previously demonstrated that the expression of the human CALM/AF10 fusion gene in murine bone marrow stem and progenitor cells results in an aggressive acute myeloid leukemia in vivo. In this study, we have screened the various domains essential for CALM-AF10 function and leukemogenicity. Our study identifies a mutant of CALM-AF10 that greatly enhances the clonogenic potential of hematopoietic progenitors while retaining key characteristics of disease induced by the full length CALM-AF10 fusion.
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| Overall design |
Global gene expression of bone marrow cells transduced with various constructs were compared. We used the empty vector, MIG, as a control and baseline. Four samples are tested with three biological replicates each.
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| Contributor(s) |
Deshpande AJ, Rouhi A, Lin Y, Stadler C, Greif P, Arseni N, Opatz S, Quintanilla-Fend L, Holzmann K, Hiddemann W, Xu G, Armstrong S, Bohlander SK, Buske C |
| Citation(s) |
21681188 |
| Submission date |
Feb 24, 2011 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Karlheinz Holzmann |
| E-mail(s) |
karlheinz.holzmann@uni-ulm.de
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| Organization name |
Ulm University
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| Department |
Medical Faculty
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| Lab |
Genomics Core Facility
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| Street address |
Helmholtzstreet 8/1
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| City |
Ulm |
| ZIP/Postal code |
D-89081 |
| Country |
Germany |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (12)
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| GSM679707 |
Bone marrow transduced with MIG, biological replicate-1 |
| GSM679708 |
Bone marrow transduced with MIG, biological replicate-2 |
| GSM679709 |
Bone marrow transduced with MIG, biological replicate-3 |
| GSM679710 |
Bone marrow transduced with CALM-AF10, biological replicate-1 |
| GSM679711 |
Bone marrow transduced with CALM-AF10, biological replicate-2 |
| GSM679712 |
Bone marrow transduced with CALM-AF10, biological replicate-3 |
| GSM679713 |
Bone marrow transduced with CALM-AF10 ΔOM-LZ, biological replicate-1 |
| GSM679714 |
Bone marrow transduced with CALM-AF10 ΔOM-LZ, biological replicate-2 |
| GSM679715 |
Bone marrow transduced with CALM-AF10 ΔOM-LZ, biological replicate-3 |
| GSM679716 |
Bone marrow transduced with CALM(400-648)+AF10 (677-758), biological replicate-1 |
| GSM679717 |
Bone marrow transduced with CALM(400-648)+AF10 (677-758), biological replicate-2 |
| GSM679718 |
Bone marrow transduced with CALM(400-648)+AF10 (677-758), biological replicate-3 |
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| This SubSeries is part of SuperSeries: |
| GSE27514 |
Identification of a Potently Oncogenic CALM-AF10 Minimal-Fusion Mutant |
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| Relations |
| BioProject |
PRJNA141749 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE27513_RAW.tar |
50.5 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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