|
| Status |
Public on May 31, 2011 |
| Title |
Expression data from parthenogenetic and WT iPS samples and their parental fibroblasts |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Three parthenogenetic induced pluripotent stem cell (PgHiPSCs) lines were generated from each of the ovarian teratoma cell lines (two distinct individuals). Two normal iPS cell lines were generated from normal fibroblasts. Three biological replicates of normal embryonic stem cells (H9, HESCs) were perfomed.
We used microarrays to study the gene expression profiles of the PgHiPSCs, and compared the expression of genes to both embryonic and induced pluripotent stem cell, to identify paternally expressed genes that are down-regulated in the PgHiPSC lines.
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| |
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| Overall design |
All parthenogenetic and normal iPS cell lines, were tested for pluripotency assays (inclusing, morphology, immuno stanings and qRT-PCR for known pluripotency markers, differentiation capacity in vivo and in vitro)
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| |
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| Contributor(s) |
Benvenisty N, Stelzer Y |
| Citation(s) |
21572443, 24371806 |
| Submission date |
Feb 16, 2011 |
| Last update date |
Aug 22, 2019 |
| Contact name |
Yonatan Stelzer |
| E-mail(s) |
yonatan.stelzer@gmail.com
|
| Phone |
972-2-6586774
|
| Fax |
972-2-6584972
|
| Organization name |
The Hebrew University of Jerusalem
|
| Department |
Genetics
|
| Lab |
Prof. Nissim Benvenisty Stem Cell Unit
|
| Street address |
Givat-Ram
|
| City |
Jerusalem |
| ZIP/Postal code |
91904 |
| Country |
Israel |
| |
|
| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (14)
|
| GSM676315 |
iPS from fibroblast A - cell line 11 |
| GSM676316 |
Fibroblast B |
| GSM676317 |
iPS from fibroblast B - cell line 36 |
| GSM676318 |
iPS from fibroblast B - cell line 41 |
| GSM676319 |
iPS from fibroblast B - cell line 34 |
| GSM676320 |
normal fibroblast |
| GSM676321 |
iPS from WT fibroblast - cell line 1 |
| GSM676322 |
iPS from WT fibroblast - cell line 2 |
| GSM676323 |
H9 embryonic stem cell - biological replicate 1 |
| GSM676324 |
H9 embryonic stem cell - biological replicate 2 |
| GSM676325 |
H9 embryonic stem cell - biological replicate 3 |
|
| Relations |
| BioProject |
PRJNA137029 |
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