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| Status |
Public on May 20, 2024 |
| Title |
Human CD127 negative ILC2s show immunological memory. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Group 2 Innate Lymphoid Cells (ILC2s) are key players in type 2 immunity and contribute to maintaining homeostasis and responding to inflammation. ILC2s are implicated in the development of type 2 inflammation-mediated chronic disorders like asthma. While memory ILC2s have been identified in mouse, it is unknown whether human ILC2s can acquire immunological memory. Here, we demonstrate the persistence of CD45RO, a marker previously linked to inflammatory ILC2s, in resting ILC2s that have undergone prior activation. A high proportion of these cells concurrently reduce the expression of the canonical ILC marker CD127 in a tissue specific manner. Through isolation and in vitro stimulation of CD127- CD45RO+ ILC2s, we observed an augmented ability to proliferate and produce cytokines. CD127- CD45RO+ ILC2s are found in both healthy and inflamed tissues and display a gene signature of cell activation. Our findings suggest that human ILC2s can acquire innate immune memory and warrants a revision of the current strategies to identify human ILC2s.
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| Overall design |
Single cell sequencing of human dermal CD45+ Lin- cells
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| Contributor(s) |
Martinez-Høyer S, Heesters BA, Spits H, Martinez-Gonzalez I |
| Citation(s) |
38889332 |
| |
| Submission date |
May 09, 2024 |
| Last update date |
Aug 20, 2024 |
| Contact name |
Itziar Martinez Gonzalez |
| Organization name |
Karolinska Institute
|
| Street address |
Biomedicum C7, Solnavägen 9
|
| City |
Solna |
| ZIP/Postal code |
171 65 |
| Country |
Sweden |
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| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
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| Samples (1) |
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| Relations |
| BioProject |
PRJNA1109755 |
