|
| Status |
Public on May 07, 2024 |
| Title |
lncRNA profile of PANC-1 treated with CAFs derived EVs |
| Organism |
Homo sapiens |
| Experiment type |
Non-coding RNA profiling by high throughput sequencing
|
| Summary |
Perineural invasion (PNI) is a unique biological feature of pancreatic cancer and is a key cause of pancreatic cancer metastasis, recurrence and poor postoperative survival, but its mechanism is largely unclarified. Clinical sample analysis and endoscopic ultrasonographic elasticity scoring indicated that cancer-associated fibroblasts (CAFs) are closely related to the occurrence of PNI. Furthermore, CAF-derived extracellular vesicles played an extremely important role in PNI in a dorsal root ganglion (DRG) coculture model and sciatic nerve model. To explore the lncRNA packaged in CAFs EVs transmitted to PANC-1, we conducted the lncRNA profile of PAN-1 treated with CAFs derived EVs.
|
| |
|
| Overall design |
CAFs were isolated from pancreatic cancer and conditional medium were collected for EVs isolation. PANC-1 were treated with CAF-derived EVs and then the RNA were extracted for lncRNA-seq.
|
| |
|
| Contributor(s) |
Zheng S, Hu C, Ting T, Chen R |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 06, 2024 |
| Last update date |
May 07, 2024 |
| Contact name |
Shangyou Zheng |
| E-mail(s) |
zhengshy23@mail.sysu.edu.cn
|
| Phone |
+8615013228635
|
| Organization name |
Guangdong Provincial People's Hospital
|
| Street address |
Guangzhou
|
| City |
Guangzhou |
| State/province |
Guangdong |
| ZIP/Postal code |
5100080 |
| Country |
China |
| |
|
| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
|
| Samples (6)
|
|
| Relations |
| BioProject |
PRJNA1108213 |
Less...
More...