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Status |
Public on May 13, 2024 |
Title |
Effect of AsiDNA and belinostat on gene expression in U87 cells. [RNA-Seq] |
Organism |
Homo sapiens |
Experiment type |
Expression profiling by high throughput sequencing
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Summary |
An effective long-term inhibition of multiple DNA repair signals is required to design a novel and effective therapeutic for glioblastoma (GBM), a highly DNA repair ‘addicted’ cancer. AsiDNA, a double-strand DNA break (DSB) mimetic, is an innovative approach that shuts down the entire DNA repair system in cancer cells by sequestering DSB repair factors. We showed that inhibition of class I histone deacetylases (HDACs) dislodges repair factors from sites of DNA damage. We therefore tested whether AsiDNA and pan HDAC inhibitor Belinostat combination can impart a chromatin-based long-term DNA damage and impair DNA repair in GBM cells. In order to understand the long-term effects of AsiDNA on DNA repair, we treated U87 cells with AsiDNA for 6 days followed by 6 days recovery from the drug. We used this protocol to create long-term AsiDNA treated cells (LTAU87) according to the clinical trial protocol. We performed RNA-Seq with LTAU87 cells and MTU87 (mock treated U87; control) in the presence and absence of belinostat.
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Overall design |
Comparative gene expression analysis was performed following exposure of glioblastoma cells to AsiDNA and belinostat.
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Contributor(s) |
Bhaskara S, Chandrasekharan M |
Citation missing |
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Submission date |
May 05, 2024 |
Last update date |
May 13, 2024 |
Contact name |
Mahesh Babu Chandrasekharan |
E-mail(s) |
mahesh.chandrasekharan@hci.utah.edu
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Phone |
801-213-4220
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Organization name |
University of Utah SOM
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Department |
Radiation Oncology
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Lab |
HCI Chandrasekharan Lab
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Street address |
2000 circle of hope room 3715
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City |
salt lake city |
State/province |
Utah |
ZIP/Postal code |
84112 |
Country |
USA |
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Platforms (1) |
GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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Samples (8)
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Relations |
BioProject |
PRJNA1107963 |