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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 16, 2012 |
| Title |
miR-148b modulates the expression of multiple genes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
To identify which miR-148b targets were involved in tumorigenesis, a microarray analysis was performed for miR-148b over-expressing cells versus controls and 129 (49 up and 80 down) modulated genes were revealed.
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| Overall design |
The effects of miR-148b on cancer progression depend on the direct and indirect regulation of multiple target genes. To identify miR-148b modulated genes, MDA-MB-231 cells were transfected with miR-148b precursors or negative controls (pre-miR-148b or control) and used 48h later for microarray and western blot (WB) analyses. When a “Whole Human Genome Oligo Microarray” (Agilent) platform was employed, 129 differentially expressed genes (49 upregulated, 80 downmodulated) were found at 48h, considering a fold change (FC) cut of 1.5 and a false discovery rate (FDR) of 16% (Table S4).
Crossing these results with the list of putative miR-148b targets (3642) obtained by the miRecords System, we observed that 33 of the modulated genes were also miR-148b predicted targets and interestingly, 26 out of these 33 genes were downmodulated.
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| Contributor(s) |
Cimino D, De Pittà C, Zampini M, Casara S, Orso F, Pinatel E, Romualdi C, Ponzone R, Brisken C, Provero P, De Bortoli M, Sismondi P, Lanfranchi G, Taverna D |
| Citation(s) |
23233531 |
| Submission date |
Jan 18, 2011 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Gerolamo Lanfranchi |
| E-mail(s) |
stefano.cagnin@unipd.it
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| Phone |
+39-0498276219
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| Organization name |
University of Padova
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| Department |
CRIBI - Biotechnology Center and Biology Department
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| Lab |
Functional Genomics Lab
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| Street address |
Via U. Bassi, 58/B
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| City |
Padova |
| ZIP/Postal code |
35131 |
| Country |
Italy |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (8)
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| GSM656334 |
CTR-A (CTR-1, biological replicate 1) |
| GSM656335 |
CTR-B (CTR-2, biological replicate 2) |
| GSM656338 |
CTR-C (CTR-2+, biological replicate 3) |
| GSM656340 |
CTR-D (CTR-3, biological replicate 4) |
| GSM656342 |
miR-148b-A (miR-148b-1, biological replicate 1) |
| GSM656343 |
miR-148b-B (miR-148b-1+, biological replicate 2) |
| GSM656348 |
miR-148b-C (miR-148b-2+, biological replicate 3) |
| GSM656349 |
miR-148b-D (miR-148b-3, biological replicate 4) |
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| This SubSeries is part of SuperSeries: |
| GSE26666 |
miR148b is a major coordinator of breast cancer progression in a relapse-associated microRNA signature by targeting ITGA5, ROCK1, PIK3CA, NRAS and CSF1 |
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| Relations |
| BioProject |
PRJNA142189 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE26662_RAW.tar |
62.8 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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