 |
 |
GEO help: Mouse over screen elements for information. |
|
| Status |
Public on May 03, 2024 |
| Title |
Cytoadhesion of Plasmodium falciparum-infected red blood cells changes the expression of cytokine-, histone- and antiviral protein-encoding genes in brain endothelial cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
Introduction Malaria remains a significant global health problem, particularly due to the human malaria parasite Plasmodium falciparum, which is responsible for most fatal infections. Infected red blood cells (iRBCs) evade spleen clearance by adhering to endothelial cells (ECs), triggering capillary blockage, inflammatory cytokine release, endothelial dysfunction, and altered vascular permeability, prompting an endothelial transcriptional response. Methods The iRBCIT4var04/HBEC-5i model, where iRBCs present IT4var04 (VAR2CSA) on their surface was employed to analyse the effects of iRBC binding on ECs. We used this model to investigate how cytoadhesion of iRBCs to ECs influences their expression profile depending on the temperature (37°C vs 40°C). Results Binding of non-infected RBCs (niRBCs) and fever alone significantly changes expression of hundreds of genes in ECs. Comparing the expression profile of HBEC-5i cultured either in the presence of iRBCs or in the presence of niRBCs, genes encoding proteins assigned to the GO terms immune response, nucleosome assembly, NF-kappa B signaling, angiogenesis, and antiviral immune response/interferon-alpha/beta signaling pathway were significantly up-regulated. If the cultivation temperature is increased from 37°C to 40°C, which simulates fever, a further significant increase in expression can be observed for most regulated genes, especially for genes coding for cytokines and proteins involved in angiogenesis. Conclusion The presence of iRBCs leads to the stimulation of ECs, activating several immunological signaling pathways and affecting antiviral (-parasitic) mechanisms and angiogenesis. Furthermore, to our knowledge, the induction of the interferon-alpha/beta signaling pathway in ECs in response to iRBCs has been described for the first time.
|
| |
|
| Overall design |
The iRBCIT4var04/HBEC-5i model, where Plasmodium falciparum infected red blood cells (iRBC) present IT4var04 (VAR2CSA) on their surface was employed to analyse the effects of iRBC binding on endothelial cells (ECs). We used this model to investigate how cytoadhesion of iRBCs to ECs influences their expression profile depending on the temperature (37°C vs 40°C).
|
| |
|
| Contributor(s) |
Allweier J, Bartels M, Torabi H, Martinez MT, Metwally N, Roeder T, Gutsmann T, Bruchhaus I |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 01, 2024 |
| Last update date |
May 03, 2024 |
| Contact name |
Johannes Allweier |
| E-mail(s) |
johannes.allweier@bnitm.de
|
| Organization name |
Bernhard Nocht Institute for Tropical Medicine
|
| Street address |
Bernhard-Nocht-Straße 74
|
| City |
Hamburg |
| ZIP/Postal code |
20359 |
| Country |
Germany |
| |
|
| Platforms (1) |
| GPL20301 |
Illumina HiSeq 4000 (Homo sapiens) |
|
| Samples (15)
|
|
| Relations |
| BioProject |
PRJNA1106906 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE266399_ExpressionBrowser.xlsx |
26.1 Mb |
(ftp)(http) |
XLSX |
SRA Run Selector |
| Raw data are available in SRA |
|
|
|
|
 |
Less...
More...