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| Status |
Public on May 03, 2024 |
| Title |
The tissue-resident regulatory T cell pool is shaped by transient multi-tissue migration and a conserved residency program. scRNA-Seq profiling of mouse tissue Tregs with TCR sequencing and CITE-Seq |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
Other
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| Summary |
The tissues are the site of many important immunological reactions, yet how the immune system is controlled at these sites remains opaque. Recent studies have identified Foxp3+ regulatory T cells (Tregs) in non-lymphoid tissues, with unique characteristics compared to lymphoid Tregs. However, tissue Tregs have not been considered holistically across tissues. Here we performed a systematic analysis of the Treg population residing in non-lymphoid organs throughout the body, revealing shared phenotypes, transient residency and common molecular dependencies. Tissue Tregs from different non-lymphoid organs shared T cell receptor (TCR) sequences, with functional capacity to drive multi-tissue Treg entry, and were tissue-agnostic on tissue homing. Together these results demonstrate that the tissue-resident Treg pool in most non-lymphoid organs, other than the gut, is largely constituted by broadly self-reactive Tregs, characterised by transient multi-tissue migration. This work suggests common regulatory mechanisms may allow pan-tissue Tregs to safeguard homeostasis across the body.
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| Overall design |
scRNA-Seq was performed using 10x Genomics 5’ VDJ Single Cell Immune Profiling. Tregs were flow sorted on a BD Influx, Aria, Jazz or Fusion on the basis of CD4+Foxp3Thy1.1+ i.v.CD45- CD19-CD11b-CD8-F4/80-. Cells were labeled with Hashtag TotalSeq regents and loaded onto the 10x Chromium Controller. Sequencing was performed on an Illumina HiSeq. Data was processed in R using scripts as detailed below.
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| Contributor(s) |
Liston A, Burton OT, Gergelits V, Bricard O |
| Citation missing |
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| Submission date |
Apr 29, 2024 |
| Last update date |
May 03, 2024 |
| Contact name |
Adrian Liston |
| E-mail(s) |
al989@cam.ac.uk
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| Organization name |
University of Cambridge
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| Department |
Department of Pathology
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| Lab |
Liston-Dooley Lab
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| Street address |
Tennis Court Road
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| City |
Cambridge |
| ZIP/Postal code |
CB2 1QP |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
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| Samples (12)
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| GSM8240877 |
Mouse 1, pooled barcoded tissue Tregs, GEX |
| GSM8240878 |
Mouse 1, pooled barcoded tissue Tregs, VDJ |
| GSM8240879 |
Mouse 1, pooled barcoded tissue Tregs, FB |
| GSM8240880 |
Mouse 2, pooled barcoded tissue Tregs, GEX |
| GSM8240881 |
Mouse 2, pooled barcoded tissue Tregs, VDJ |
| GSM8240882 |
Mouse 2, pooled barcoded tissue Tregs, FB |
| GSM8240883 |
Mouse 3, pooled barcoded tissue Tregs, GEX |
| GSM8240884 |
Mouse 3, pooled barcoded tissue Tregs, VDJ |
| GSM8240885 |
Mouse 3, pooled barcoded tissue Tregs, FB |
| GSM8240886 |
Mouse 4, pooled barcoded tissue Tregs, GEX |
| GSM8240887 |
Mouse 4, pooled barcoded tissue Tregs, VDJ |
| GSM8240888 |
Mouse 4, pooled barcoded tissue Tregs, FB |
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| Relations |
| BioProject |
PRJNA1105902 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE266111_RAW.tar |
83.2 Mb |
(http)(custom) |
TAR (of CSV, MTX, TSV) |
SRA Run Selector |
| Raw data are available in SRA |
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