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| Status |
Public on May 03, 2024 |
| Title |
Tetrastigma hemsleyanum Polysaccharides Alleviate Imiquimod-Induced Psoriasis-Like Skin Lesions in Mice by Modulating the JAK/STAT3 Signaling Pathway |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Crosstalk between keratin-forming cells and aberrant immune cells due to immune imbalance is a key factor in the pathogenesis of psoriasis. Most of the current clinical treatments provide rapid symptomatic relief but bring side effects that should not be ignored. Tetrastigma hemsleyanum polysaccharides (THP) have significant immunomodulatory and anti-inflammatory properties. In this study, we used imiquimod (IMQ)-induced psoriasis mouse model and LPS/IL-6-stimulated HaCaT cell model. The potential and mechanism of action of THP for psoriasis treatment were assessed by Psoriasis Area Severity Index (PASI) score, histopathology, flow cytometry, Western blot, and reverse transcription-polymerase chain reaction. The signs and symptoms of psoriatic mice induced by IMQ were significantly relieved by percutaneous administration of THP, including the improvement of psoriatic skin signs (erythema, folds, scales), pathological changes, decreased PASI score, and decreased spleen index. Results of in vivo and in vitro studies suggest that THP inhibits abnormal cell proliferation and excessive inflammation at skin lesions, balances Th17 immune cells, and blocks the keratinocyte-Th17 cell cycle, thus playing a role in psoriasis treatment by a mechanism that may be related to the regulation of the JAK/STAT3 signaling pathway.
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| Overall design |
After 1 week of adaptation, mice were randomly divided into two experimental groups: (1) Control group (CON); (2) IMQ group (MOD). Except for the negative control group, each mouse received local treatment with 62.5 mg of IMQ on the shaved dorsal skin for 6 consecutive days. At the end of the experiment, mice were intramuscularly injected with 0.02 mg/mL atropine (0.02 mL/10 g), intraperitoneally injected with 6.5 mg/mL zoledronic acid (0.1 mL/10 g), blood was collected via cardiac puncture, and euthanasia was performed by cervical dislocation. A portion of the skin tissue was fixed in 10% formalin, while another portion was rapidly frozen in liquid nitrogen and stored at -80°C for subsequent analysis. Spleen tissue was weighed, with a portion placed in pre-chilled phosphate-buffered saline (PBS) for flow cytometry analysis, and the remaining portion fixed in 10% formalin.
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| Contributor(s) |
Lv Y, Yang L, Zhou F, Ye Y |
| Citation missing |
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| Submission date |
Apr 28, 2024 |
| Last update date |
May 03, 2024 |
| Contact name |
Yishan Lv |
| E-mail(s) |
shaowen.liu@majorbio.com
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| Organization name |
Zhejiang Chinese Medical University
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| Street address |
548 Binwen Road
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| City |
hanzhou |
| ZIP/Postal code |
310053 |
| Country |
China |
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| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA1105570 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE266043_gene.TPM.matrix.annot.xls.gz |
8.9 Mb |
(ftp)(http) |
XLS |
SRA Run Selector |
| Raw data are available in SRA |
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