|
| Status |
Public on Dec 29, 2010 |
| Title |
Expression data from xenograft tumors derived from LoVo colon cancer lines +/- constitutive LIN28B expression |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
We sought to elucidate functions of LIN28B and potential mechanisms whereby it may promote metastasis by comparing the gene expression profile of LIN28B metastases to primary tumors. Accordingly, we performed microarray analysis on total RNA isolated from empty vector tumors, LIN28B-LoVo tumors, and LIN28B-LoVo metastases
|
| |
|
| Overall design |
Constitutive LIN28B expression was achieved in the LoVo (ATCC #CCL-229) colon cancer cell line via retroviral transduction of MSCV-PIG-LIN28B.
xenografts were produced via injection of 1x106 cells subcutaneously into the rear flanks of nude mice. Microarrays were conducted on primary tumors from empty vector
and LIN28B-expressing cells, as well as metastases derived from primary tumors constitutively expressing LIN28B. (Note: metastases did not occur with empty vector tumors)
|
| |
|
| Contributor(s) |
King C, Rustgi A |
| Citation(s) |
21512136 |
| Submission date |
Dec 28, 2010 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Catrina King |
| E-mail(s) |
catrinak@vet.upenn.edu
|
| Phone |
215-573-7314
|
| Organization name |
University of Pennsylvania
|
| Department |
Gastroenterology
|
| Lab |
Rustgu
|
| Street address |
415 Curie Blvd
|
| City |
Philadelphia |
| State/province |
PA |
| ZIP/Postal code |
19139 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (9)
|
| GSM646700 |
vector-LoVo xenograft tumor biological replicate 1 |
| GSM646701 |
vector-LoVo xenograft tumor biological replicate 2 |
| GSM646702 |
vector-LoVo xenograft tumor biological replicate 3 |
| GSM646703 |
Lin28b-LoVo xenograft tumor biological replicate 1 |
| GSM646704 |
Lin28b-LoVo xenograft tumor biological replicate 2 |
| GSM646705 |
Lin28b-LoVo xenograft tumor biological replicate 3 |
| GSM646706 |
Lin28b-LoVo xenograft metastasis biological replicate 1 |
| GSM646707 |
Lin28b-LoVo xenograft metastasis biological replicate 2 |
| GSM646708 |
Lin28b-LoVo xenograft metastasis biological replicate 3 |
|
| This SubSeries is part of SuperSeries: |
|
| Relations |
| BioProject |
PRJNA142245 |
Less...
More...