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GEO help: Mouse over screen elements for information. |
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| Status |
Public on Dec 17, 2011 |
| Title |
Expression data from in vitro and ex vivo Th1- and Th17-polarized TRP1 TCR transgenic CD4+ cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Serial comparison between Th1 and Th17 tumor-specific cells cultured in vitro and ex vivo after transferred into sublethaly irradiated B6.PL mice. Th17-derived cells acquire Th1-like properties in vivo but maintain a distinct molecular profile.
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| Overall design |
TRP1 CD4+ T cells were peptide stimulated under Th1 and Th17 conditions for 7 days. mRNA was collected on the day of adoptive transfer and from multiple replicates on day 5 and 15 after adoptive transfer from pooled spleens and inguinal lymph nodes. Tissues were enriched for CD4 by negative selection and sorted by flow cytometry for the expression of Thy1.2.
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| Contributor(s) |
Muranski P, Kern SJ, Ferreyra GA, Borman ZA, Danner RL, Restifo NP |
| Citation(s) |
22177921 |
| Submission date |
Dec 13, 2010 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Steven Kern |
| E-mail(s) |
kernsj@cc.nih.gov
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| Phone |
301-435-2683
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| Organization name |
National Institutes of Health
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| Department |
Critical Care Medicine Department
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| Street address |
10 Center Drive
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| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (28)
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| GSM639264 |
Th1 ex vivo day 5, replicate 2 |
| GSM639265 |
Th1 ex vivo day 5, replicate 3 |
| GSM639266 |
Th17 ex vivo day 5, replicate 1 |
| GSM639267 |
Th17 ex vivo day 5, replicate 2 |
| GSM639268 |
Th17 ex vivo day 5, replicate 3 |
| GSM639269 |
Th1 ex vivo day 15, replicate 1 |
| GSM639270 |
Th1 ex vivo day 15, replicate 2 |
| GSM639271 |
Th17 ex vivo day 15, replicate 1 |
| GSM639272 |
Th17 ex vivo day 15, replicate 2 |
| GSM639273 |
Th17 ex vivo day 15, replicate 3 |
| GSM639274 |
Th17 ex vivo day 15, replicate 4 |
| GSM639275 |
Th1 day 0 in vitro, 2hr stim |
| GSM639276 |
Th17 day 0 in vitro, 2hr stim |
| GSM639277 |
Th1 ex vivo day 5, replicate 1, 2hr stim |
| GSM639278 |
Th1 ex vivo day 5, replicate 2, 2hr stim |
| GSM639279 |
Th1 ex vivo day 5, replicate 3, 2hr stim |
| GSM639280 |
Th17 ex vivo day 5, replicate 1, 2hr stim |
| GSM639281 |
Th17 ex vivo day 5, replicate 2, 2hr stim |
| GSM639282 |
Th17 ex vivo day 5, replicate 3, 2hr stim |
| GSM639283 |
Th1 ex vivo day 15, replicate 1, 2hr stim |
| GSM639284 |
Th1 ex vivo day 15, replicate 2, 2hr stim |
| GSM639285 |
Th17 ex vivo day 15, replicate 1, 2hr stim |
| GSM639286 |
Th17 ex vivo day 15, replicate 2, 2hr stim |
| GSM639287 |
Th17 ex vivo day 15, replicate 3, 2hr stim |
| GSM639288 |
Th17 ex vivo day 15, replicate 4, 2hr stim |
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| Relations |
| BioProject |
PRJNA135495 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE26030_RAW.tar |
119.3 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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