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Series GSE25910 Query DataSets for GSE25910
Status Public on Aug 13, 2012
Title Adipose Tissue MicroRNAs as Regulators of CCL2 Production in Human Obesity (differentiation data)
Organism Homo sapiens
Experiment type Expression profiling by array
Summary We used an unbiased systems biology approach to study the regulation of gene expression in human adipose tissue focusing on inflammation. We show that microRNAs play a major role as regulators of CCL2 production in obesity.
 
Overall design Subcutaneous adipose tissue was obtained from healthy subjects undergoing cosmetic liposuction (n=12). There was no selection for age, sex or BMI. From the tissue, we isolated preadipocytes and in vitro differentiated them to adipocytes. The cells were lysed at day 4/5 (early), 8 (middle) and 12 (late) of differentiation. From the samples, we prepared and hybridised biotinylated complementary RNA to GeneChip Human Gene 1.0 ST Arrays (Affymetrix Inc., Santa Clara, CA). Pre-processing was performed using the Affymetrix Expression Console version 1.1 and the following settings: Summarization: PLIER; Background Correction: PM-GCBG; Normalization: Global Median.
 
Contributor(s) Mejhert N, Arner E, Dahlman I, Arner P
Citation(s) 22688341, 24439387, 24379347, 27346647, 29348496, 31383551
Submission date Dec 07, 2010
Last update date Sep 03, 2019
Contact name Ingrid Dahlman
E-mail(s) ingrid.dahlman@ki.se
Organization name Karolinska
Street address Karolinska Huddinge
City Stockholm
ZIP/Postal code 14186
Country Sweden
 
Platforms (1)
GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
Samples (36)
GSM636662 In vitro differentiated adipocytes_subject 74_day 4/5
GSM636663 In vitro differentiated adipocytes_subject 74_day 8
GSM636664 In vitro differentiated adipocytes_subject 74_day 12
This SubSeries is part of SuperSeries:
GSE25402 Adipose Tissue MicroRNAs as Regulators of CCL2 Production in Human Obesity
Relations
BioProject PRJNA142455

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE25910_RAW.tar 139.0 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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