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| Status |
Public on Jan 01, 2012 |
| Title |
Human Beta-Defensin 3 attenuates the pro-inflammatory effects of LPS at 1 hour |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
We examine the global effect of hBD3 on transcription in TLR4-stimulated macrophages and for the first time show that hBD3 inhibits the transcription of critical pro-inflammatory genes.
Among the repressed genes we detect significant enrichment of groups involved in the positive regulation of NFkappaB including components of Toll-like receptor signaling pathways.
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| Overall design |
Bone marrow derived macrophages from 3 separate mice were used for each treatment condition. ie macrophages from 3 mice were left untreated, macrophages from 3 mice were treated with LPS, macrophages from 3 mice were treated with LPS and hBD3, macrophages from 3 mice were treated with hBD3. We examined the differences in gene expression between each treatment group.
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| Contributor(s) |
Semple F, Dorin JR, Webb S, Grimes GR, Semple CA |
| Citation(s) |
21809339 |
| Submission date |
Dec 06, 2010 |
| Last update date |
Aug 14, 2019 |
| Contact name |
Graeme Richard Grimes |
| E-mail(s) |
graeme.grimes@ed.ac.uk
|
| Organization name |
University of Edinburgh, The Institute of Genetics and Cancer
|
| Department |
Human Genetics Unit
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| Lab |
Bioinformatics Analysis Core
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| Street address |
Western General Hospital, Crewe Road
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| City |
Edinburgh |
| ZIP/Postal code |
EH4 2XU |
| Country |
United Kingdom |
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| Platforms (1) |
| GPL6885 |
Illumina MouseRef-8 v2.0 expression beadchip |
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| Samples (12)
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| This SubSeries is part of SuperSeries: |
| GSE25873 |
Effect of hBD3 on transcription in TLR4-stimulated macrophages |
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| Relations |
| BioProject |
PRJNA142471 |
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