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| Status |
Public on Dec 01, 2010 |
| Title |
Sorted B cells from mice treated with MPL and/or R837 |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
Many successful vaccines induce persistent antibody responses that can last a lifetime. The mechanisms by which they do so remain unclear, but emerging evidence suggests that activate dendritic cells (DCs) via Toll-like receptors (TLRs). For example, the yellow fever vaccine YF-17D, one of the most successful empiric vaccines ever developed, activates DCs via multiple TLRs to stimulate pro-inflammatory cytokines. Triggering specific combinations of TLRs in DCs can induce synergistic production of cytokines, which results in enhanced T cell responses, but its impact on antibody responses remain unknown. Learning the critical parameters of innate immunity that programs such antibody responses remains a major challenge in vaccinology. We demonstrated that immunization of mice with synthetic nanoparticles containing antigens plus Toll-like receptor (TLR) ligands 4 (MPL) + 7 (R837) induces synergistic increases in antigen-specific, neutralizing antibodies compared to immunization with a single TLR ligand. To determine whether there was any early programming of B cells, we isolated isotype switched, TCRbeta-CD11b-CD19+IgD-IgG+ B cells by FACS at 7 days post immunization with nanoparticles containing various adjuvants plus OVA, and performed microarray analyses to assess their molecular signatures.
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| Overall design |
Two independent sets of samples at day 7 post-treatment were used in our analyses. Each set is comprised by B-cells from mice treated with MPL + R837 or from those treated with either individual MPL or R837 alone.
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| Contributor(s) |
Kasturi SP, Skountzou I, Nakaya HI, Pulendran B |
| Citation(s) |
21350488 |
| Submission date |
Nov 29, 2010 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Helder I. Nakaya |
| E-mail(s) |
hnakaya@usp.br
|
| Phone |
+551126481130
|
| Organization name |
Universidade de São Paulo
|
| Lab |
csbiology.com
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| Street address |
AVENIDA PROFESSOR LINEU PRESTES, 580, Block 17
|
| City |
SÃO PAULO |
| State/province |
SÃO PAULO |
| ZIP/Postal code |
05508000 |
| Country |
Brazil |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
|
| GSM630822 |
B cells from mice treated with MPL - rep 1 |
| GSM630823 |
B cells from mice treated with R837 - rep 1 |
| GSM630824 |
B cells from mice treated with MPL and R837 - rep 1 |
| GSM630825 |
B cells from mice treated with MPL - rep 2 |
| GSM630826 |
B cells from mice treated with R837 - rep 2 |
| GSM630827 |
B cells from mice treated with MPL and R837 - rep 2 |
|
| Relations |
| BioProject |
PRJNA133875 |
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