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| Status |
Public on May 11, 2024 |
| Title |
Identifying the transcriptomic effects of tRNA-induced proline-to-serine mistranslation in Drosophila melanogaster |
| Organism |
Drosophila melanogaster |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Mistranslation describes the misincorporation of an amino acid into a nascent polypeptide. Mistranslation has diverse effects on multicellular eukaryotes and is implicated in several human diseases. We introduced a mistranslating serine transfer RNA (tRNA) into Drosophila melanogaster that misincorporates serine at proline codons and found that it affected male and female flies differently. Here, we compare the transcriptomic response of male and female flies to mistranslation to identify cellular pathways that underlie this sex-specific response. Both males and females downregulated genes associated with metabolism in response to proline-to-serine mistranslation. Only males downregulated genes associated with developmental processes and response to negative stimuli such as infection, whereas only females downregulated aerobic respiration and ATP synthesis genes. Both sexes upregulated genes associated with gametogenesis but females upregulated cell cycle and DNA maintenance genes, suggesting that mistranslation may compromise genome integrity in females. This transcriptomic analysis advances our understanding of how males and females respond to mistranslation and has important implications for future studies that examine the influence of mistranslation on disease.
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| Overall design |
RNA sequencing performed on 10 male or female flies containing an additional wildtype serine tRNA with a UGA anticodon (FlyBase ID: FBgn0050201) or an additional serine tRNA modified to contain a UGG anticodon and a G26A mutation causing misincorporation of serine at proline codons
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| Contributor(s) |
Isaacson JR, Berg MD, Yeung W, Brandl CJ, Moehring AJ |
| Citation(s) |
38766246 |
| Submission date |
Feb 22, 2024 |
| Last update date |
May 31, 2024 |
| Contact name |
Amanda Jean Moehring |
| E-mail(s) |
amoehrin@uwo.ca
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| Phone |
519-661-2111
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| Organization name |
Western University
|
| Department |
Biology
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| Lab |
Moehring Lab
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| Street address |
1151 Richmond Street, Western Science Centre 307
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| City |
London |
| State/province |
Ontario |
| ZIP/Postal code |
N6A3K7 |
| Country |
Canada |
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| Platforms (1) |
| GPL17275 |
Illumina HiSeq 2500 (Drosophila melanogaster) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA1079207 |
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