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Series GSE250032 Query DataSets for GSE250032
Status Public on May 03, 2024
Title Combination of a SOS1-KRAS interaction inhibitor with a KRASG12C inhibitor combination can address intrinsic and acquired resistance leading to stronger and more durable response (Quant-Seq 2)
Organism Homo sapiens
Experiment type Expression profiling by high throughput sequencing
Summary To investigate the transcriptomic changes associated with KRAS G12C inhibitor Adagrasib resistance and Adagrasib + SOS1 inhibitor (BI-3406) or Adagrasib + EGFR inhibitor (Cetuximab) combination treatment to overcome resistance.
We performed differential gene expression analysis using RNA-seq generated from cell line derived xenograft (CDX) in vivo models. We compared different treatment conditions with DMSO treated condition.
 
Overall design CDX models were generated using NSCLC cell line NCI-H2122 and CRC cell line SW837. For each CDX category, expression data with DMSO treated and either single agent and combination treatment was generated.
Repetition of the original experiment. Includes technical replicates for samples previously included and new samples for additional time points
 
Contributor(s) Thatikonda V, Gerlach D, Bergner O, Alpar D, Hofmann M, Kamuda M
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Submission date Dec 12, 2023
Last update date May 04, 2024
Contact name Daniel Gerlach
E-mail(s) daniel.gerlach@boehringer-ingelheim.com
Organization name Boehringer Ingelheim RCV GmbH & Co KG
Department Global Computational Biology and Digital Sciences
Street address Dr.-Boehringer-Gasse 5-11
City Vienna
ZIP/Postal code 1121
Country Austria
 
Platforms (1)
GPL18573 Illumina NextSeq 500 (Homo sapiens)
Samples (65)
GSM7971630 NCI-H2122 cells, Vehicle_A1
GSM7971631 NCI-H2122 cells, Vehicle_A2
GSM7971632 NCI-H2122 cells, Vehicle_A3
This SubSeries is part of SuperSeries:
GSE225061 Combination of a SOS1-KRAS interaction inhibitor with a KRASG12C inhibitor combination can address intrinsic and acquired resistance leading to stronger and more durable response
Relations
BioProject PRJNA1051636

Download family Format
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Supplementary file Size Download File type/resource
GSE250032_RAW.tar 282.2 Mb (http)(custom) TAR (of TSV)
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Raw data are available in SRA
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