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| Status |
Public on May 22, 2011 |
| Title |
Expression profiling of host genes modulated by Epstein-Barr virus Rta in nasopharyngeal carcinoma cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
EBV Rta is a transcriptional activator that functions to disrupt EBV latency in cells of epithelial origin. This series of experiment is to identify host genes that are moduated by the expression of doxycycline-inducible EBV Rta in nasopharyngeal carcinoma cells. Designations for the two EBV Rta inducible cell lines are TW01TetER_cl7 (lower expression level) and TW01TetER_cl19 (higher expression level); for the control line is TW01Tet.
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| Overall design |
All the three inducible cell lines were grown to log-phase before doxycycline induction. Same number of cells from the three cell lines were treated with doxycycline for 24 h. Total RNAs from the three samples were extracted and subjected to microarray analysis (Affymetrix Human Gene 1.0 ST Array , n=33,297). We sought to identify genes up- or down-regulated in the two doxycycline-treated EBV Rta cells, but not in the treated control cells.
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| Contributor(s) |
Lin S, Chen Y, Tsai W, Chen Y, Ko Y |
| Citation(s) |
21423768, 21918011 |
| Submission date |
Oct 07, 2010 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Su-Fang Lin |
| E-mail(s) |
sflin1@gmail.com
|
| Organization name |
National Health Research Institutes, Taiwan
|
| Department |
NICR
|
| Lab |
R2-1211
|
| Street address |
No 35, Keyan Road, Zhunan Town
|
| City |
Miaoli Conunty |
| ZIP/Postal code |
35053 |
| Country |
Taiwan |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (3) |
| GSM606067 |
Control, doxcycline 24 h, rep 1 |
| GSM606068 |
TW01TetER_cl7, doxycycline 24 h, rep1 |
| GSM606069 |
TW01TetER_cl19, doxycycline 24 h, rep1 |
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| This SubSeries is part of SuperSeries: |
| GSE24587 |
Epstein–Barr virus (EBV) Rta-mediated cell cycle arrest enables permissive replication of EBV and Kaposi’s sarcoma-associated herpesvirus in 293 cells |
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| Relations |
| BioProject |
PRJNA133585 |
