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| Status |
Public on May 07, 2024 |
| Title |
Binding Changes in Chromatin Factors Upon Targeted XPO1 Inhibition in T Cells |
| Organism |
Homo sapiens |
| Experiment type |
Other
Genome binding/occupancy profiling by high throughput sequencing
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| Summary |
We assessed the chromatin localization of XPO1 and p300 in response to C646 (10 uM), S109 (1 uM), and SP100030 (1 uM).
We used CUT&RUN sequencing as a lower-input (500,000 cells per sample), higher-sensitivity alternative to ChIP-Seq to assess chromatin binding of different factors
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| Overall design |
Differential binding analysis and overlap analysis of XPO1 and p300 at basal conditions (DMSO), activated conditions (50 nM PMA + 1 uM Ionomycin), or activated + small molecule treatment in Jurkat T cells
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| Contributor(s) |
Chen Y, Adams DJ |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Sep 30, 2023 |
| Last update date |
May 07, 2024 |
| Contact name |
Yi Fan Chen |
| E-mail(s) |
yxc1272@case.edu
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| Organization name |
Case Western Reserve University School of Medicine
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| Street address |
2109 Adelbert Road Biomedical Research Building 747A
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| City |
Cleveland |
| State/province |
OH |
| ZIP/Postal code |
44106 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (20)
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| GSM7814686 |
Jurkat cells, 24 hrs, IgG control antibody 1 |
| GSM7814687 |
Jurkat cells, 24 hrs, XPO1 antibody |
| GSM7814688 |
Jurkat cells, 24 hrs, C646, XPO1 antibody |
| GSM7814689 |
Jurkat cells, 24 hrs, p300 antibody |
| GSM7814690 |
Jurkat cells, 24 hrs, C646, p300 antibody |
| GSM7814691 |
Jurkat cells, 24 hrs, IgG control antibody 2 |
| GSM7814692 |
Jurkat cells, 8 hrs, XPO1 antibody |
| GSM7814693 |
Jurkat cells, 8 hrs hours, S109, XPo1 antibody |
| GSM7814694 |
Jurkat cells, 24 hours, p300 antibody |
| GSM7814695 |
Jurkat cells, 24 hours, C646, p300 antibody |
| GSM7814696 |
Jurkat cells, 8 hrs, S109, p300 antibody |
| GSM7876476 |
Jurkat XPO1 C528S cells, 24 hrs, IgG control antibody [IgG_C528S] |
| GSM7876477 |
Jurkat XPO1 C528S cells, 24 hrs, XPO1 antibody [XPO1_C528S] |
| GSM7876478 |
Jurkat XPO1 C528S cells, 24 hrs, 10 uM C646, XPO1 antibody [XPO1_C646_C528S] |
| GSM7876479 |
Jurkat XPO1 C528S cells, 24 hrs, p300 antibody [p300_C528S] |
| GSM7876480 |
Jurkat XPO1 C528S cells, 24 hrs, 10 uM C646, p300 antibody [p300_C646_C528S] |
| GSM8119566 |
Jurkat cells, basal state (no activation), 8 hours, p300 antibody, rep1 |
| GSM8119567 |
Jurkat cells, basal state (no activation), 8 hours, p300 antibody, rep2 |
| GSM8119568 |
Jurkat cells, PMA Ionomycin, 8 hours, p300 |
| GSM8119569 |
Jurkat cells, PMA Ionomycin + 1 uM SP100030, 8 hours, p300 |
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| Relations |
| BioProject |
PRJNA1022781 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE244396_RAW.tar |
2.1 Gb |
(http)(custom) |
TAR (of BIGWIG) |
SRA Run Selector |
| Raw data are available in SRA |
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