|
| Status |
Public on Nov 15, 2011 |
| Title |
human duodenum biopsy data |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Using a systems biology approach, we discovered and dissected a three-way interaction between the immune system, the intestinal epithelium, and the microbiota. We found that mice lacking B lymphocytes, or lacking IgA, have low intestinal expression of lipid metabolism genes regulated by the transcription factor GATA4, and a consequent decrease in fat absorption in the intestine. The defect disappeared in germ free mice, suggesting that it is dependent on the microbiota; and sequencing analysis of the bacteria showed subtle differences between normal and B-cell deficient mice. Analysis of gene expression of gut biopsies from patients with common variable immunodeficiency and intestinal dysfunction revealed a high similarity to mouse B-cell knockout profiles. These data provide an explanation for a longstanding enigmatic association between immunodeficiency and defective lipid absorption in humans.
this series represents the subsection of the study where we analize gene epxression in duodenum biopsies from CVID patients and contols with unrelated pathologies
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| |
|
| Overall design |
Reference sample is from normal duodenum (Clontech). Log2 ratio Cy5/Cy3 was used.
|
| |
|
| Contributor(s) |
Shulzhenko N, Morgun A |
| Citation(s) |
22101768 |
| Submission date |
Sep 01, 2010 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Natalia Shulzhenko |
| E-mail(s) |
anemorgun@hotmail.com, shulzhenkon@niaid.nih.gov
|
| Organization name |
NIAID, USA
|
| Department |
Laboratory of Cellular and Molecular Immunology
|
| Lab |
Ghost lab
|
| Street address |
9000 Rockville Pike
|
| City |
Bethesda |
| State/province |
MD |
| ZIP/Postal code |
20892 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
|
| Samples (6)
|
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| This SubSeries is part of SuperSeries: |
| GSE23934 |
Crosstalk between B lymphocytes, microbiota and the intestinal epithelium governs immunity versus metabolism in the gut |
|
| Relations |
| BioProject |
PRJNA133205 |
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