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| Status |
Public on Jul 27, 2023 |
| Title |
Gain-of-function genetic perturbationssimultaneouslyacross500 barcoded cancer cell lines |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
Using thisapproach, wequeriedthe pan-cancer vulnerability landscape upon activating 10key pathway nodes, revealing selectiveactivation dependenciesof MAPK and PI3K pathwaysassociatedwith specific biomarkers
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| Overall design |
To profile activation of the curated gene sets across a broad panel of cell lines, we developed a high-throughput method for systematically examining the growth-suppressive effect of ORF expression in ~500 cancer cell lines. We next tested the perturbation effects of 11 ORFs (including GFP as a negative control) by lentiviral infection of each ORF encoding virus into separate pools of 488 cell lines.
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| Contributor(s) |
Chang L, Rees M, Roth J, Ronan M, Kocak M |
| Citation(s) |
37749246 |
| Submission date |
Jul 24, 2023 |
| Last update date |
Oct 16, 2023 |
| Contact name |
Sellers Lab |
| Organization name |
Broad Institute
|
| Street address |
415 Main St
|
| City |
Cambridge |
| State/province |
MA |
| ZIP/Postal code |
02142 |
| Country |
USA |
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| Platforms (1) |
| GPL16791 |
Illumina HiSeq 2500 (Homo sapiens) |
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| Samples (66)
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| Relations |
| BioProject |
PRJNA997999 |
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