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| Status |
Public on May 17, 2024 |
| Title |
Increased activity of protein kinase A, even without DNAJB1, is sufficient to cause Fibrolamellar hepatocellular carcinoma |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Most fibrolamellar carcinoma (FLC) is driven by a fusion of DNAJB1 and PRKACA, the catalytic subunit of protein kinase A (PKA). Overexpression of DNAJB1::PRKACA, ATP1B1::PRKACA or PRKACA, but not catalytically inactive kinase, caused similar transcriptomic changes of primary human hepatocytes; these recapitulated most changes observed in FLC. This is consistent with the observation that FLC is found in patients missing a regulatory subunit or with a ATP1B1::PRKACA fusion. Thus, the DNAJB1 domain is not required for FLC.
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| Overall design |
Primary Human Hepatocytes were transduced with either DNAJB1::PRKACA, ATP1B1::PRKACA, PRKACA or kinase inactive DNAJB1::PRKACA or dsRED.
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| Contributor(s) |
Requena D, Shirani M, deJong YP, Simon SM |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jul 18, 2023 |
| Last update date |
May 17, 2024 |
| Contact name |
David Requena |
| E-mail(s) |
drequena@rockefeller.edu
|
| Phone |
(917) 293-6642
|
| Organization name |
Rockefeller University
|
| Lab |
Laboratory of Cellular Biophysics
|
| Street address |
1230 York Avenue
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10065 |
| Country |
USA |
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| Platforms (1) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
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| Samples (15)
|
| GSM7643721 |
Transduced with ATP1B1::PRKACA sample 1 |
| GSM7643722 |
Transduced with ATP1B1::PRKACA sample 2 |
| GSM7643723 |
Transduced with ATP1B1::PRKACA sample 3 |
|
| Relations |
| BioProject |
PRJNA996178 |
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