|
| Status |
Public on May 14, 2024 |
| Title |
Inflammatory Alterations to Renal Lymphatic Endothelial Cell Gene Expression in Mouse Models of Hypertension |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
To evaluate the specific changes that renal LECs undergo in hypertension (HTN), we performed single-cell RNA sequencing. Using the angiotensin II-induced and salt-sensitive mouse models of HTN, we isolated renal CD31+ and podoplanin+ cells from hypertensive and normotensive mice. Sequencing of these cells revealed three distinct cell types (LECs, myeloid immune cells, and an unusual third population we dubbed multipotent nephron-associated support cells) with unique expression profiles.
|
| |
|
| Overall design |
Mice underwent the angiotensin-II and salt sensitive models of hypertension and had their kidneys removed and aggregated per group (A2 HTN, A2 control, SS HTN, SS control). Kidneys were digested and turned to a single-cell suspension, followed by the isolation of CD31+ and podoplanin+ cells by magnetic separation. We sought to investigate the effects of hypertension on CD31+/podoplanin+ renal lymphatic endothelial cells and to investigate model-specific differences.
|
| |
|
| Contributor(s) |
McDermott JG, Goodlett BL, Creed HA, Navaneethabalakrishnan S, Rutkowski JM, Mitchell BM |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Jul 03, 2023 |
| Last update date |
May 14, 2024 |
| Contact name |
Brett Mitchell |
| Organization name |
Texas A&M University
|
| Department |
Medical Physiology
|
| Lab |
Mitchell
|
| Street address |
8441 Riverside Pkwy
|
| City |
Bryan |
| State/province |
TX |
| ZIP/Postal code |
77807 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL24247 |
Illumina NovaSeq 6000 (Mus musculus) |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA990767 |
Less...
More...