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| Status |
Public on May 01, 2024 |
| Title |
Microarray analysis of regenerating parietal cells (PCs) in murine gastric epithelium recovering from Diphtheria toxin injury |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
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| Summary |
Highly specialized, acid-secreting PCs are one of many epithelial lineages that line the gastric epithelium. Even though PCs are functionally and structurally distinct from other lineages in the stomach, the molecular mechanisms that govern their specification and maturation from the gastric stem cells are relatively unknown. We used microarray analysis to interrogate transcriptional changes that occur in regenerating PCs and to identify candidate regulators of PC fate specification and maturation.
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| Overall design |
Transgenic Atp4b-DTR mice were treated daily with diphtheria toxin for 3 days to induce reversible PC ablation. The gastric corpus was harvested at 24, 36, 48, or 72 hours after the final injection for the experimental groups and from vehicle treated mice for the uninjured control sample. Two independent samples were collected for 48 and72h timepoints; all others had 1 sample. The corpus was collected, snap frozen, then RNA isolated using Qiagen RNeasy Micro Kit and qulality assesed with Nanodrop.
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| Contributor(s) |
Mills JC, Bark S, Adkins-Threats M |
| Citation missing |
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| Submission date |
Jun 12, 2023 |
| Last update date |
May 01, 2024 |
| Contact name |
Jason C Mills |
| E-mail(s) |
Jason.Mills@bcm.edu
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| Phone |
713-798-4882
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| Organization name |
Baylor College of Medicine
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| Street address |
1 Baylor Plaza
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| City |
Houston |
| State/province |
Texas |
| ZIP/Postal code |
77030 |
| Country |
USA |
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| Platforms (1) |
| GPL16570 |
[MoGene-2_0-st] Affymetrix Mouse Gene 2.0 ST Array [transcript (gene) version] |
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| Samples (7)
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| Relations |
| BioProject |
PRJNA982884 |
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