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| Status |
Public on Aug 05, 2010 |
| Title |
In vivo gene expression changes in human blood cells in response to radiation therapy. |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Our in vivo study measures miRNA and gene expression changes in human blood cells in response to ionizing radiation in order to develop miRNA signatures that can be used as biomarkers for radiation exposure. Blood was collected from 8 radiotherapy patients immediately prior to and at 4 h after total body irradiation with 1.25 Gy X-rays. Both miRNA and gene expression changes were measured by means of quantitative PCR and microarray hybridization, respectively. Patients were in complete remission at the time of blood collection. Out of 223 differentially expressed genes, 37 were both downregulated and predicted targets of the upregulated miRNAs. Both miRNA and gene control of biological processes such as hemopoiesis and the immune response is increased after irradiation, whereas metabolic processes are underrepresented among all differentially expressed genes and the genes controlled by miRNAs.
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| Overall design |
Sixteen human whole-blood samples were included in this study. Eight samples were collected shortly before total body irradiation. The other 8 samples were collected at 4 h after total body irradiation with 1.25 Gy X-rays.
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| Contributor(s) |
Paul S, Amundson SA, Templin T, Smilenov LB |
| Citation(s) |
21420249 |
| Submission date |
Aug 03, 2010 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Thomas Templin |
| E-mail(s) |
tt2308@columbia.edu
|
| Phone |
212-305-5661
|
| Fax |
212-305-3229
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| URL |
http://www.crr-cu.org/templin.htm
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| Organization name |
Columbia University Medical Center
|
| Street address |
630 West 168th Street
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (16)
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| Relations |
| BioProject |
PRJNA131289 |
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