|
| Status |
Public on Jul 28, 2011 |
| Title |
Expression data from WT and GABPalpha-deficient Flt3(-) LSK cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
GABPalpha is an Ets family transcription factor and involved in regulation of both basic cellular functions such as cell cycle progression and tissue-specific biological processes. We found that GABPalpha is critically required for survival and differentiation of hematopoietic stem cells.
We used microarrays to detect gene expression changes in Flt3(-) LSK cells (which contains both long-term and short-term hematopoietic stem cells) by GABPalpha deficiency.
|
| |
|
| Overall design |
Mx1Cre-GABPa(FL/+) and Mx1Cre-GABPa(FL/-) mice were treated with pIpC to induce inactivation of GABPalpha in bone marrow cells. Four days after last treatment of pIpC, the bone marrow cells were isolated and the Flt3(-)LSK subset was purified by cell sorting. RNA was extracted and hybridized to GeneChip Mouse GENE 1.0 ST arrays (Affymetrix).
|
| |
|
| Contributor(s) |
Xue H, Yu S |
| Citation(s) |
21139080 |
| Submission date |
Jul 30, 2010 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Hai-Hui Xue |
| E-mail(s) |
haihui.xue@hmh-cdi.org
|
| Organization name |
Hackensack University Medical Center
|
| Department |
Center for Discovery and Innovation
|
| Street address |
111 Ideation Way
|
| City |
Nutley |
| State/province |
NJ |
| ZIP/Postal code |
07110 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
|
| Samples (4)
|
|
| Relations |
| BioProject |
PRJNA131479 |
Less...
More...