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| Status |
Public on Feb 20, 2024 |
| Title |
Effect of global translation initiation inhibition associated to glucose starvation on cytoplasmic lncRNAs levels |
| Organism |
Saccharomyces cerevisiae |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To analyze the effect of global translation initiation inhibition associated to glucose deprivation on cytoplasmic lncRNAs levels, we performed RNA-Seq using WT, xrn1-delta and upf1-delta cells grown in glucose-containing complete synthetic medium (CSM) and then shifted for 16 min in glycerol- and ethanol-containing medium. In parallel, control cells were maintained for the same time in glucose-containing CSM. For the WT strains, we also included a treatment of 15 min with CHX (100 μg/ml final concentration) or an equal volume of DMSO (Mock).
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| Overall design |
Strand-specific total RNA-seq (2 biological replicates per condition).
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| Contributor(s) |
ANDJUS S, SZACHNOWSKI U, VOGT N, HATIN I, PAPADOPOULOS C, LOPES A, NAMY O, WERY M, MORILLON A |
| Citation(s) |
38443115 |
| Submission date |
May 19, 2023 |
| Last update date |
Mar 06, 2024 |
| Contact name |
Maxime Wery |
| E-mail(s) |
maxime.wery@curie.fr
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| Organization name |
Institut Curie
|
| Department |
UMR3244
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| Lab |
Non-coding RNA, epigenetics and genome fluidity
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| Street address |
26 rue d'Ulm
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| City |
Paris |
| ZIP/Postal code |
75005 |
| Country |
France |
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| Platforms (1) |
| GPL27812 |
Illumina NovaSeq 6000 (Saccharomyces cerevisiae) |
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| Samples (16)
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| This SubSeries is part of SuperSeries: |
| GSE203283 |
Pervasive translation of Xrn1-sensitive unstable long non-coding RNAs in yeast |
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| Relations |
| BioProject |
PRJNA974380 |
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