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| Status |
Public on Oct 26, 2023 |
| Title |
SCR106 splicing factor modulates abiotic stress responses by maintaining the RNA splicing in rice |
| Organism |
Oryza sativa Japonica Group |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We investigated the function of the plant-specific SR protein SC106 in pre-mRNA splicing regulation and abiotic stress responses in rice. The loss-of-function mutant, SC106, showed increased sensitivity to salt. We performed genome-wide analyses of gene expression and splicing in seedlings subjected to salt stress and identified multiple splice isoforms from stress-responsive genes dependent on SC106.
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| Overall design |
7-day-old seedlings of sc106 mutant, and WT germinated on 125mM NaCl and mock conditions. . The RNA-seq libraries were constructed using TruSeq mRNA stranded kit following the standard protocol and sequenced on the Nova-seq platform to generate high-quality paired-end reads.
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| Web link |
http://10.1093/jxb/erad433
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| |
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| Contributor(s) |
Alhabsi A, Butt H, Blilou I, Mahfouz MM |
| Citation(s) |
37924151 |
| Submission date |
May 10, 2023 |
| Last update date |
Jan 27, 2024 |
| Contact name |
Haroon Butt |
| E-mail(s) |
haroon.butt.1@kaust.edu.sa
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| Phone |
00966542529194
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| Organization name |
KAUST
|
| Department |
BESE
|
| Lab |
Laboratory for Genome Engineering and Synthetic Biology
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| Street address |
King Abdullah University of Science and Technology (KAUST) Thuwal, 23955-6900
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| City |
Jeddah |
| State/province |
Makkah |
| ZIP/Postal code |
4700 |
| Country |
Saudi Arabia |
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| Platforms (1) |
| GPL27860 |
Illumina NovaSeq 6000 (Oryza sativa Japonica Group) |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA971147 |
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