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| Status |
Public on May 15, 2024 |
| Title |
RNA-seq analysis of B cells cultured with LPS and IL-12 and/or IFN gamma |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Purpose: We aimed to find genes differentially regulated by IL-12 and IFNg alone, and in combination, over 4 and 24 hours of stimulation.
Methods : Spleens were processed and B cell cultures prepared, in triplicate, according to the manuscript. After the indicated times, cells were washed in PBS, resuspended in 350 ul RLT Plus Buffer (Qiagen) with 1% 2-Me, and frozen at -80 ⁰C.
Results: At 4 hours, the main effect of IL-12 is to induce IFNg production from B cells, whereas IFNg induces many gene changes. Comparing 24 hours to the 4 hour stimulation, IFNg alone was insufficient to maintain the majority of IFNg-induced 4 hour changes, while adding IL-12 sustained a portion of the 4 hour gene changes. Additionally, in combination IL-12 and IFNg cooperate to induce unique gene changes that are not shared with either the individual cytokines at either timepoint.
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| Overall design |
We used recombinant IL-12 and IFNg or anti-IL-12 and anti-IFNg blocking antibodies to assess the contribution of each cytokine alone and in combination.
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| Contributor(s) |
Elsner RA, Smita S, Shlomchik MJ |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
May 08, 2023 |
| Last update date |
May 15, 2024 |
| Contact name |
Mark Shlomchik |
| E-mail(s) |
shlomchiklab@gmail.com
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| Organization name |
University of Pittsburgh
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| Department |
Department of Immunology
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| Street address |
The Assembly, 5051 Centre Avenue
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| City |
Pittsburgh |
| State/province |
PA |
| ZIP/Postal code |
15213 |
| Country |
USA |
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| Platforms (1) |
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| Samples (42)
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| GSM7306190 |
4 hours, IL-12 at 2000 pg/mL, IFNg block, mouse 1 |
| GSM7306191 |
4 hours, IL-12 at 2000 pg/mL, IFNg block, mouse 2 |
| GSM7306192 |
4 hours, IL-12 at 2000 pg/mL, IFNg block, mouse 3 |
| GSM7306193 |
4 hours, IL-12 at 2000 pg/mL, IFNg at 0.5 ng/mL, mouse 1 |
| GSM7306194 |
4 hours, IL-12 at 2000 pg/mL, IFNg at 0.5 ng/mL, mouse 2 |
| GSM7306195 |
4 hours, IL-12 at 2000 pg/mL, IFNg at 0.5 ng/mL, mouse 3 |
| GSM7306196 |
4 hours, IL-12 at 200 pg/mL, IFNg at 0.5 ng/mL, mouse 1 |
| GSM7306197 |
4 hours, IL-12 at 200 pg/mL, IFNg at 0.5 ng/mL, mouse 2 |
| GSM7306198 |
4 hours, IL-12 at 200 pg/mL, IFNg at 0.5 ng/mL, mouse 3 |
| GSM7306199 |
4 hours, IL-12 at 200 pg/mL, IFNg block, mouse 1 |
| GSM7306200 |
4 hours, IL-12 at 200 pg/mL, IFNg block, mouse 2 |
| GSM7306201 |
4 hours, IL-12 at 200 pg/mL, IFNg block, mouse 3 |
| GSM7306202 |
4 hours, IL-12 at 20 pg/mL, IFNg at 0.5 ng/mL, mouse 1 |
| GSM7306203 |
4 hours, IL-12 at 20 pg/mL, IFNg at 0.5 ng/mL, mouse 2 |
| GSM7306204 |
4 hours, IL-12 at 20 pg/mL, IFNg at 0.5 ng/mL, mouse 3 |
| GSM7306205 |
4 hours, IL-12 at 20 pg/mL, IFNg block, mouse 1 |
| GSM7306206 |
4 hours, IL-12 at 20 pg/mL, IFNg block, mouse 2 |
| GSM7306207 |
4 hours, IL-12 at 20 pg/mL, IFNg block, mouse 3 |
| GSM7306208 |
24 hours, IL-12 at 20 pg/mL, IFNg at 0.5 ng/mL, mouse 1 |
| GSM7306209 |
24 hours, IL-12 at 20 pg/mL, IFNg at 0.5 ng/mL, mouse 2 |
| GSM7306210 |
24 hours, IL-12 at 20 pg/mL, IFNg at 0.5 ng/mL, mouse 3 |
| GSM7306211 |
24 hours, IL-12 at 20 pg/mL, IFNg block, mouse 1 |
| GSM7306212 |
24 hours, IL-12 at 20 pg/mL, IFNg block, mouse 2 |
| GSM7306213 |
24 hours, IL-12 at 20 pg/mL, IFNg block, mouse 3 |
| GSM7306214 |
24 hours, IL-12 block, IFNg at 0.5 ng/mL, mouse 1 |
| GSM7306215 |
24 hours, IL-12 block, IFNg at 0.5 ng/mL, mouse 2 |
| GSM7306216 |
24 hours, IL-12 block, IFNg at 0.5 ng/mL, mouse 3 |
| GSM7306217 |
24 hours, IL-12 block, IFNg block, mouse 1 |
| GSM7306218 |
24 hours, IL-12 block, IFNg block, mouse 2 |
| GSM7306219 |
24 hours, IL-12 block, IFNg block, mouse 3 |
| GSM7306220 |
4 hours, IL-12 block, IFNg at 0.5 ng/mL, mouse 1 |
| GSM7306221 |
4 hours, IL-12 block, IFNg at 0.5 ng/mL, mouse 2 |
| GSM7306222 |
4 hours, IL-12 block, IFNg at 0.5 ng/mL, mouse 3 |
| GSM7306223 |
4 hours, IL-12 block, IFNg at 3 ng/mL, mouse 1 |
| GSM7306224 |
4 hours, IL-12 block, IFNg at 3 ng/mL, mouse 2 |
| GSM7306225 |
4 hours, IL-12 block, IFNg at 3 ng/mL, mouse 3 |
| GSM7306226 |
4 hours, IL-12 block, IFNg block, mouse 1 |
| GSM7306227 |
4 hours, IL-12 block, IFNg block, mouse 2 |
| GSM7306228 |
4 hours, IL-12 block, IFNg block, mouse 3 |
| GSM7306229 |
4 hours, no added IL-12 nor blocked, IFNg at 3 ng/mL, mouse 1 |
| GSM7306230 |
4 hours, no added IL-12 nor blocked, IFNg at 3 ng/mL, mouse 2 |
| GSM7306231 |
4 hours, no added IL-12 nor blocked, IFNg at 3 ng/mL, mouse 3 |
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| Relations |
| BioProject |
PRJNA970259 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE231931_RAW.tar |
3.0 Mb |
(http)(custom) |
TAR (of TXT) |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
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