|
| Status |
Public on May 17, 2024 |
| Title |
Generation of immune-enhancing neutrophils by subclinical super-low dose LPS |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We have generated immune-enhancing neutrophils by culturing murine primary bone marrow derived neutrophils with either super-low dose of LPS. Immune-enhancing neutrophils preferentially express co-stimulatory molecules such as CD74, CD44 and CD86, and exhibit reduced expression of CD11b. Purified bone marrow neutrophils were treated with PBS or 100 pg/ml LPS overnight in the presence of GM-CSF, and harvested for scRNAseq analysis to examine their profiles of gene expression.
|
| |
|
| Overall design |
WT murine neutrophils were stimulated by GM-CSF, and then treated with either PBS or LPS overnight
|
| |
|
| Contributor(s) |
Zhang Y, YI Z, Geng S, Li L |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 21, 2023 |
| Last update date |
May 18, 2024 |
| Contact name |
Liwu Li |
| E-mail(s) |
lwli@vt.edu
|
| Organization name |
Virginia Tech
|
| Street address |
1015 Life Science Cir
|
| City |
Blacksburg |
| State/province |
VA |
| ZIP/Postal code |
24060 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL21103 |
Illumina HiSeq 4000 (Mus musculus) |
|
| Samples (2) |
|
| Relations |
| BioProject |
PRJNA958103 |
