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| Status |
Public on Apr 15, 2023 |
| Title |
Transcriptome studies reveal the regulation of m6A in testis development and spermatogenesis in yak |
| Organism |
Bos grunniens |
| Experiment type |
Expression profiling by high throughput sequencing
Methylation profiling by high throughput sequencing
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| Summary |
Studying spermatogenesis is the key to understanding the development mechanism of the yak reproductive system. Although N6-methyladenosine (m6A) RNA modification has been reported to regulate spermatogenesis and reproductive function in mammals, the molecular mechanism of m6A in yak testis development and spermatogenesis remains largely unknown. Therefore, we collected testicular tissue from junile and adult yaks, and found that the m6A level significantly increased after sexual maturity in yak. In MeRIP-seq, 1,702 hypermethylated peaks and 724 hypomethylated peaks were identified. The hypermethylated differentially methylated RNAs (DMRs) (CIB2, AK1, FOXJ2, PKDREJ, SLC9A3, and TOPAZ1) mainly regulated spermatogenesis, while the hypomethylated DMRs (BCL6, USP25, CD96, EPHA2, and TAF12) mainly participated in immune response. Functional enrichment analysis showed that DMRs were significantly enriched in the adherens junction, gap junction, and Wnt, PI3K, and mTOR signaling pathways, regulating cell development, spermatogenesis, and testicular endocrine function. The functional analysis of differentially expressed genes showed that they were involved in the biological processes of mitosis, meiosis, and flagellated sperm motility during sexual maturity of yak testis. We also screened the key regulatory factors of testis development and spermatogenesis by combined analysis, which included BRCA1, CREBBP, STAT3, and SMAD4. This study has important research value and practical guiding significance for yak genetic improvement, particularly in understanding the molecular mechanism and evolutionary dynamics of the progression of testis developmental stages in yaks.
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| Overall design |
Detection of m6A in yak testis before and after sexual maturity
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| Contributor(s) |
Guo S, Wang X, Pei J, Guo X |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Apr 11, 2023 |
| Last update date |
Apr 17, 2023 |
| Contact name |
shaoke guo |
| E-mail(s) |
82101205332@caas.cn
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| Phone |
15036009308
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| Organization name |
Chinese Academy of Agricultural Sciences
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| Department |
Lanzhou institute of husbandry and pharmaceutical sciences
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| Street address |
West Lake Street
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| City |
Lanzhou |
| State/province |
Gansu |
| ZIP/Postal code |
730050 |
| Country |
China |
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| Platforms (1) |
| GPL30409 |
Illumina NovaSeq 6000 (Bos grunniens) |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA954275 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE229363_Y30M_m6A_site.txt.gz |
1.8 Mb |
(ftp)(http) |
TXT |
| GSE229363_Y6M-vs-Y30M-m6A.txt.gz |
682.3 Kb |
(ftp)(http) |
TXT |
| GSE229363_Y6M_Y30M_fpkm.txt.gz |
668.5 Kb |
(ftp)(http) |
TXT |
| GSE229363_Y6M_m6A_site.txt.gz |
19.8 Mb |
(ftp)(http) |
TXT |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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