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| Status |
Public on Nov 10, 2010 |
| Title |
Effect of T350 phosphorylation on gene repression activity of EZH2 in BJ cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
The Polycomb group (PcG) protein enhancer of zeste homolog 2 (EZH2) plays an essential role in histone methylation-mediated epigenetic gene silencing. We demonstrate that under physiological conditions, cyclin-dependent kinase 1 (CDK1) and 2 (CDK2) bind to and phosphorylate EZH2 at threonine 350 (T350) in an evolutionally conserved motif. T350 phosphorylation is required for EZH2 function in silencing of genes that promote differentiation and inhibit proliferation and migration. Blockage of T350 phosphorylation by generating a T350A mutation largely diminishes the global effect of EZH2 on gene silencing.
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| Overall design |
Four experiments have been designed. BJ cells were transfected with si-Control, si-EZH2, si-EZH2 plus RNAi resistant lentivirus expressed EZH2, or si-EZH2 plus RNAi resistant lentivirus expressed T350A mutant for 60 hours. Each experiment was triplicated.
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| Contributor(s) |
Chen S, Huang H |
| Citation(s) |
20935635 |
| Submission date |
Jun 17, 2010 |
| Last update date |
Aug 13, 2018 |
| Contact name |
Shuai Chen |
| E-mail(s) |
chenx495@umn.edu
|
| Organization name |
University of Minnesota
|
| Department |
Cancer Center
|
| Street address |
MMC 806, 420 Delaware Street S.E.
|
| City |
Minneapolis |
| State/province |
MN |
| ZIP/Postal code |
55455 |
| Country |
USA |
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| Platforms (1) |
| GPL10558 |
Illumina HumanHT-12 V4.0 expression beadchip |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA128549 |
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