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| Status |
Public on Sep 17, 2010 |
| Title |
Identification and characterization of two novel primate-specific histone H3 variants H3.X and H3.Y |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Nucleosomal incorporation of specialized histone variants is an important mechanism to generate different functional chromatin states. Here we report the identification and characterization of two novel primate-specific histone H3 variants, H3.X and H3.Y. Their mRNAs are found in certain human cell lines, in addition to several normal and malignant human tissues. In keeping with their primate-specificity, H3.X and H3.Y are detected in different brain regions. Transgenic H3.X and H3.Y proteins are stably incorporated into chromatin in a similar fashion to the known H3 variants. Importantly, we demonstrate biochemically and by mass spectrometry that endogenous posttranslationally modified H3.Y protein exists in vivo, and that stress-stimuli, such as starvation and cellular density, increase the abundance of H3.Y-expressing cells. Global transcriptome analysis revealed that knock-down of H3.Y affects cell growth and leads to changes in the expression of many genes involved in cell cycle control. Thus, H3.Y is a novel histone variant involved in the regulation of cellular responses to outside stimuli.
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| Overall design |
Total RNA samples from human U2OS cells. Transcript levels after luciferase, H3.X and/or H3.Y RNAi was analyzed.
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| Contributor(s) |
Wiedemann SM, Mildner SN, Bönisch C, Israel L, Maiser A, Matheisl S, Straub T, Merkl R, Leonhardt H, Kremmer E, Schermelleh L, Hake SB |
| Citation(s) |
20819935 |
| Submission date |
Jun 17, 2010 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Tobias Straub |
| E-mail(s) |
tstraub@med.uni-muenchen.de
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| Organization name |
LMU Munich
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| Department |
Biomedical Center, Bioinformatics
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| Street address |
Großhadener Str. 9
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| City |
Martinsried |
| ZIP/Postal code |
82152 |
| Country |
Germany |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (6)
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| Relations |
| BioProject |
PRJNA128575 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE22415_RAW.tar |
22.6 Mb |
(http)(custom) |
TAR (of CEL) |
| Processed data included within Sample table |
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