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| Status |
Public on Aug 30, 2023 |
| Title |
VEGF overexpressed mesoangioblasts enhance urethral and vaginal recovery following simulated vaginal birth in rats |
| Organism |
Rattus norvegicus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Background: Vaginal birth causes injury to the pelvic floor and may lead to urinary incontinence. Cell therapy has been proposed to assist in functional recovery. Objective: To assess if intra-arterial injection of mesoangioblasts (MABs) or Vascular Endothelial Growth Factor (VEGF)-overexpressed MABs, improves urethral and vaginal function recovery after simulated vaginal delivery (SVD). Results: All stem-cell injected rats had external urethral sphincteric and vaginal function recovery within 14d, as compared to half of controls. Functional recovery was paralleled by improved muscle regeneration and microvascularization. Recovery was not different between autologous and allogenic MABs. MABsallo-VEGF accelerated functional recovery and increased GAP-43 expression at 7d. We detected early on in the recovery major transcriptional changes in both MABsallo and MABsallo-VEGF. MABSallo upregulated transcripts that encode proteins involved in myogenesis and dendrite development, and downregulated pro-inflammatory processes MABsallo-VEGF upregulated transcripts that encode proteins involved neuron and dendrite development and downregulates genes involved in hypoxia and oxidative stress. 7d after injury, Later MABsallo-VEGF showed a decrease in oxidative and inflammatory response compared to MABSallo treated urethras. These pathways are also downregulated in animals that have recovered at 7d, compared to non-recovered animals. Conclusion: Intra-arterial injection of MABsallo-VEGF enhances neuroregeneration and accelerates functional urethral and vaginal recovery after simulated vaginal delivery. Injection of stem cells induces faster recovery of urethral and vaginal function; cells overexpressing a factor increasing blood vessel formation, were even more effective.
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| Overall design |
Female rats (n=24) were assigned to either injection of saline (control), allogeneic-MABs (MABsallo), or allogeneic-MABs overexpressing VEGF (MABsallo-VEGF).
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| Contributor(s) |
van der Veer B |
| Citation(s) |
37244975 |
| Submission date |
Jan 10, 2023 |
| Last update date |
Aug 30, 2023 |
| Contact name |
Bernard Klaas van der Veer |
| E-mail(s) |
ben.vanderveer@kuleuven.be
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| Organization name |
KU Leuven
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| Department |
Department of Development and Regeneration
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| Lab |
Laboratory for Stem Cell and Developmental Epigenetics
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| Street address |
Herestraat 49 - box 804
|
| City |
Leuven |
| State/province |
Vlaams-Brabant |
| ZIP/Postal code |
3000 |
| Country |
Belgium |
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| Platforms (1) |
| GPL22396 |
Illumina HiSeq 4000 (Rattus norvegicus) |
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| Samples (24)
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| Relations |
| BioProject |
PRJNA922532 |
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