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| Status |
Public on Apr 19, 2023 |
| Title |
Expression data from Botryococcus terribilis ethanol extract (BTEE)-treated RAW264 cells |
| Platform organism |
Mus musculus |
| Sample organism |
Mus |
| Experiment type |
Expression profiling by array
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| Summary |
Gene expression profiling reveals anti-inflammatory effects of BTEE on lipopolysaccharide (LPS)-induced murine RAW264 cells
We evaluated the pretreatment effect of BTEE on LPS-induced inflammation in RAW264 cells. Pretreatment with BTEE could significantly attenuate nitric oxide (NO) production and LPS-induced release of inflammatory mediators in RAW264 cells.
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| Overall design |
RNA was extracted using Isogen (Nippon Gene Co. Ltd., Toyama, Japan). The integrity of RNA was quantified using NanoDrop 2000 spectrophotometer (Thermo Scientific, Wilmington, DE, USA). Clariom S assay system (Thermo Fisher, Japan) was used on duplicate RNA samples of each group. In accordance with the user manual, complementary DNA (cDNA) for microarray was generated using the GeneChip WT Plus Reagent kit (Thermo Fisher Science, Japan). Samples were hybridized using Clariom S GeneChip microarray kit (Thermo Fisher Science, Japan). After washing and staining, images were captured, and then CEL data were generated using the GeneChip Scanner 3000 (Thermo Fisher Science, Japan). The raw CEL data were normalized using the transcriptome analysis console (TAC) software ver.4.0.1 following the SST-robust multichip average (SST-RMA) algorithm (Thermo Fisher Scientific, Japan).
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| Contributor(s) |
Yamamoto S |
| Citation(s) |
37047640 |
| Submission date |
Dec 23, 2022 |
| Last update date |
Apr 21, 2023 |
| Contact name |
Farhana Ferdousi |
| E-mail(s) |
farhana_ferdousi24@yahoo.com
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| Organization name |
University of Tsukuba
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| Street address |
Tennodai 1-1-1
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| City |
Tsukuba |
| State/province |
Ibaraki |
| ZIP/Postal code |
305-8575 |
| Country |
Japan |
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| Platforms (1) |
| GPL23038 |
[Clariom_S_Mouse] Affymetrix Clariom S Assay, Mouse (Includes Pico Assay) |
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| Samples (8)
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| GSM6892268 |
RAW 264 cells_BTEE pre-treatment 24h_LPS induction 6h_rep1 |
| GSM6892269 |
RAW 264 cells_BTEE pre-treatment 24h_LPS induction 6h_rep2 |
| GSM6892270 |
RAW 264 cells_No pre-treatment_LPS induction 6h_rep1 |
| GSM6892271 |
RAW 264 cells_No pre-treatment_LPS induction 6h_rep2 |
| GSM6892272 |
RAW 264 cells_BTEE pre-treatment 24h_LPS induction 17h_rep1 |
| GSM6892273 |
RAW 264 cells_BTEE pre-treatment 24h_LPS induction 17h_rep2 |
| GSM6892274 |
RAW 264 cells_No pre-treatment_LPS induction 17h_rep1 |
| GSM6892275 |
RAW 264 cells_No pre-treatment_LPS induction 17h_rep2 |
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| Relations |
| BioProject |
PRJNA915347 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE221665.xls.gz |
2.4 Mb |
(ftp)(http) |
XLS |
| GSE221665_RAW.tar |
11.1 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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