|
| Status |
Public on Dec 31, 2023 |
| Title |
circRNA sequence authenticity in live cells |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
Exogenous RNA, such as circRNA, could be edited by endogenous editors, such as C-to-U editor activation-induced deaminase and apolipoprotein B mRNA editing enzyme, catalytic polypeptide-like (AID/APOBEC), and A-to-I editor Adenosine deaminases acting on RNA (ADAR) (I: inosine, recognized as G). Editing of circRNA may interfere with IRES and Kozak sequence to initiate antigen translation, and may alter antigen products or mutate stop codons.
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| |
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| Overall design |
Using circRNA sequencing (circRNA-seq) followed by bioinformatic circRNA mapping, we monitored the sequence integrity of circRNA-SIINFEKL after transfection in live mouse bone marrow dendritic cells (BMDCs) for 24 h.
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| Contributor(s) |
Zhang Y, Zhu G, Liu J, Tyc KM |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Dec 15, 2022 |
| Last update date |
Jan 01, 2024 |
| Contact name |
Guizhi Zhu |
| Organization name |
Virginia Commonwealth University
|
| Street address |
410 N 12th St
|
| City |
Richmond |
| State/province |
VA |
| ZIP/Postal code |
23298 |
| Country |
USA |
| |
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| Platforms (1) |
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| Samples (6)
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| GSM6843676 |
BMDCs treated with circRNA for 24 h replicate 1 |
| GSM6843677 |
BMDCs treated with circRNA for 24 h replicate 2 |
| GSM6843678 |
BMDCs treated with circRNA for 24 h replicate 3 |
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| This SubSeries is part of SuperSeries: |
|
| Relations |
| BioProject |
PRJNA912729 |
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