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| Status |
Public on Jun 30, 2010 |
| Title |
NLRC5 is a transcriptional regulator of MHC class I genes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
NLRC5 is a member of the NLR family of proteins. The observation that NLRC5 is found in the nucleus prompted us to perform a gene array to identify putative target genes of NLRC5. We generated Jurkat T cell lines that stably express either the wild-type or mutant forms of NLRC5 harboring mutations in the nucleotide binding domain (NBD): Walker A (deficient in nucleotide binding), Walker B (deficient in nucleotide hydrolysis), and the combined Walker AB, carrying both mutations.
Site-directed mutagenesis was used to create the NLRC5 NBD mutants: Walker A (K234A), Walker B (E311Q), and Walker AB (both mutations).
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| Overall design |
We compared the expression profiles of Jurkat T cells expressing GFP, or the GFP-tagged wild-type, Walker A, Walker B, or Walker AB forms of NLRC5. GFP and the Walker A and Walker AB forms of NLRC5 were considered functionally inactive, while the wild-type and Walker B forms of NLRC5 were considered functionally active. These groups were used as biological replicates to assess the effect of NLRC5 activity on gene expression.
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| Contributor(s) |
Kobayashi KS, Meissner TB, Li A |
| Citation(s) |
20639463 |
| Submission date |
Jun 01, 2010 |
| Last update date |
Jul 26, 2018 |
| Contact name |
Amy Li |
| E-mail(s) |
Amy_Li@dfci.harvard.edu
|
| Organization name |
Dana Farber Cancer Institute
|
| Department |
Cancer Immunology and AIDS
|
| Lab |
Koichi S. Kobayashi Lab
|
| Street address |
44 Binney Street
|
| City |
Boston |
| State/province |
MA |
| ZIP/Postal code |
02115 |
| Country |
USA |
| |
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| Platforms (1) |
| GPL6244 |
[HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (5)
|
| GSM548568 |
Jurkat T cells expressing GFP |
| GSM548569 |
Jurkat T cells expressing GFP-NLRC5-WT |
| GSM548570 |
Jurkat T cells expressing GFP-NLRC5-Walker A |
| GSM548571 |
Jurkat T cells expressing GFP-NLRC5-Walker B |
| GSM548572 |
Jurkat T cells expressing GFP-NLRC5-Walker AB |
|
| Relations |
| BioProject |
PRJNA127483 |
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