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| Status |
Public on Dec 01, 2023 |
| Title |
Expression data from primary human healthy donor-derived CD14- cDC2s, CD14+ cDC2s and monocytes |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
The human dendritic cell (DC) family has recently been expanded by CD1c+CD14+CD163+ DCs, introduced as DC3. DC3 are found in tumors and peripheral blood of cancer patients but which cells can serve as their pre-cursors remain unknown.
CD1c+CD14+ cells share similarities with both CD1c+ DCs (cDC2s) and CD14+ monocytes on transcriptomic and phenotypic level. To investigate whether CD14+ cDC2s are closer related to CD14- cDC2s or monocytes on transcriptomic level, we analyzed their RNA.
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| Overall design |
To compare the myeloid populations on transcriptome level, RNA was isolated from FACS-sorted CD1c+CD14- DCs, CD1c+CD14+ cells and CD14+ monocytes from blood of four healthy donors and analyzed by Affymetrix microarrays.
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| Contributor(s) |
Becker AM, Florez-Grau G, Bakdash G, Roring RJ, Stelloo S, Vermeulen M, Verdoes M, De Vries IJ |
| Citation(s) |
38242119 |
| Submission date |
Nov 17, 2022 |
| Last update date |
Mar 11, 2024 |
| Contact name |
Jolanda de Vries |
| E-mail(s) |
jolanda.devries@radboudumc.nl, anouk.becker@radboudumc.nl
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| Organization name |
Radboud Institute for Molecular Life Sciences, Radboudumc
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| Department |
Tumor Immunology Department
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| Street address |
Geert grooteplein Zuid 28
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| City |
Nijmegen |
| ZIP/Postal code |
6525GA |
| Country |
Netherlands |
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| Platforms (1) |
| GPL570 |
[HG-U133_Plus_2] Affymetrix Human Genome U133 Plus 2.0 Array |
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| Samples (12)
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| Relations |
| BioProject |
PRJNA902756 |
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