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| Status |
Public on Oct 10, 2022 |
| Title |
Structure and functional mapping of the KRAB-KAP1 repressor complex [KAP1_CUT&RUN-seq] |
| Organism |
Homo sapiens |
| Experiment type |
Other
|
| Summary |
We used CUT&RUN to assess how abolition of KRAB binding by KAP1 (through structure-guided mutations in the KAP1-KRAB binding interface) changed the distribution of H3K9me3 epigenetic mark genome-wide.
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| Overall design |
Comparative H3K9me3 enirchment analysis of CUT&RUN-seq data for HEK293T cells and its derivatives [KAP1-KO, WTcomp (KAP1-KO cells complemented with a wild type KAP1), and CCmutComp (KO cells complemented with a KAP1-coiled coil mutant)]. Experiments were performed on HEK293T cells (n=2 for each condition)
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| |
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| Contributor(s) |
Pandiloski N, Douse C |
| Citation(s) |
36341546 |
| Submission date |
Oct 07, 2022 |
| Last update date |
Jan 11, 2023 |
| Contact name |
Ninoslav Pandiloski |
| E-mail(s) |
ninoslav.pandiloski@med.lu.se
|
| Organization name |
Lund University
|
| Street address |
Sölvegatan 17
|
| City |
Lund |
| ZIP/Postal code |
223 62 |
| Country |
Sweden |
| |
|
| Platforms (2) |
| GPL24676 |
Illumina NovaSeq 6000 (Homo sapiens) |
| GPL30173 |
NextSeq 2000 (Homo sapiens) |
|
| Samples (11)
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| This SubSeries is part of SuperSeries: |
| GSE215016 |
Structure and functional mapping of the KRAB-KAP1 repressor complex |
|
| Relations |
| BioProject |
PRJNA888075 |
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