|
| Status |
Public on Sep 23, 2022 |
| Title |
RNAseq of two mouse lung cancer cell lines in a DSTYK-altered context |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by high throughput sequencing
|
| Summary |
We report RNAseq data from two independent mouse syngeneic lung cancer cell lines LLC and UN-SCC679 in an altered DSTYK context
Lung cancer remains the leading cause of cancer-related death worldwide. We identify DSTYK, a dual serine/threonine and tyrosine non-receptor protein kinase, as a novel actionable target altered in non-small cell lung cancer (NSCLC). We also show DSTYK´s association with a lower overall survival (OS) and poorer progression-free survival (PFS) in multiple patient cohorts. To ascertain the potential molecular carcinogenesis processes in which DSTYK was involved, we performed an RNAseq analysis of two different lung cancer cell lines with either overexpressed or inhibited DSTYK. Inhibition was achieved through shRNA technology. These cell lines were cultured in RPMI 1640 supplemented with 10% Fetalclone (Thermo Fisher Scientific) and 100 U/mL penicillin-100 µg/mL streptomycin (Thermo Fisher Scientific). All cells were grown in a humidified incubator containing 5% CO2 at 37°C. Cell lines were routinely tested for mycoplasma.
|
| |
|
| Overall design |
Characterization of mouse syngeneic lung cancer cell lines (LLC and UN-SCC679) transcriptome in a DSTYK-altered context
|
| |
|
| Contributor(s) |
Valencia K, Montuenga LM |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Sep 07, 2022 |
| Last update date |
Sep 24, 2022 |
| Contact name |
Luis Montuenga |
| E-mail(s) |
lmontuenga@unav.es
|
| Organization name |
CIMA
|
| Street address |
Avda. Pío XII, 55
|
| City |
Pamplona |
| State/province |
Navarra |
| ZIP/Postal code |
31008 |
| Country |
Spain |
| |
|
| Platforms (1) |
|
| Samples (18)
|
|
| Relations |
| BioProject |
PRJNA877656 |
Less...
More...