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Series GSE21231 Query DataSets for GSE21231
Status Public on Aug 31, 2010
Title Gene expression changes associated with resistance to intravenous corticosteroid therapy in children with severe ulcerative colitis
Organism Homo sapiens
Experiment type Expression profiling by array
Summary Although corticosteroids remain a mainstay of therapy for UC, a meta-regression of cohort studies in acute severe ulcerative colitis (UC) showed that 29% of patients fail corticosteroid therapy and require escalation of medical management or colectomy.
We aimed to determine whether genes expressed in whole blood early following initiation of intravenous corticosteroid treatment can be associated with response.
 
Overall design 20 corticosteroid responsive (PUCAI < 35 by day 5) and 20 corticosteroid resistant patients (PUCAI ≥ 35 by day 5 and need for 2nd line medical therapy or colectomy by hospital discharge) were selected from a prospectively accrued cohort of patients hospitalized for intravenous corticosteroid treatment of severe UC. Total RNA was extracted from blood samples collected on day 3 of intravenous corticosteroid therapy. The eluted transcriptomes were quantified on Affymetrix Human Gene 1.0 ST arrays. The data was analysed by the local-pooled error method for discovery of differential gene expression and false discovery rate correction was applied to adjust for multiple comparisons. P-values less than 0.05 after correction were considered significant.
 
Contributor(s) Kabakchiev BT, Turner D, Griffiths AM, Silverberg MS
Citation(s) 20941359
Submission date Apr 06, 2010
Last update date Jul 26, 2018
Contact name Boyko Kabakchiev
Organization name Mount Sinai Hospital
Department Samuel Lunenfeld Research Institute
Lab Silverberg Lab
Street address 60 Murray Street, Room L3-004
City Toronto
State/province Ontario
ZIP/Postal code M5T 3L9
Country Canada
 
Platforms (1)
GPL6244 [HuGene-1_0-st] Affymetrix Human Gene 1.0 ST Array [transcript (gene) version]
Samples (40)
GSM530838 peripheral blood collected on day 3 from a pediatric responder to IVCS therapy SID_2372
GSM530839 peripheral blood collected on day 3 from a pediatric responder to IVCS therapy SID_2986
GSM530840 peripheral blood collected on day 3 from a pediatric responder to IVCS therapy SID_2354
Relations
BioProject PRJNA126499

Download family Format
SOFT formatted family file(s) SOFTHelp
MINiML formatted family file(s) MINiMLHelp
Series Matrix File(s) TXTHelp

Supplementary file Size Download File type/resource
GSE21231_RAW.tar 154.9 Mb (http)(custom) TAR (of CEL)
Processed data included within Sample table

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