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| Status |
Public on Jan 24, 2023 |
| Title |
The transcriptomic landscape of glutamatergic cortical neurons carrying Alzheimer’s disease PSEN1 mutations reveals alterations in structural components, signalling, and ncRNA regulation |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by high throughput sequencing
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| Summary |
To investigate gene expression changes related to two fAD mutations (A79V and L150P) in the Presenilin-1 gene (PSEN1) we compared the transcriptomes (polyA and total) of glutamatergic cortical neurons derived from fAD-mutant human induced pluripotent stem cells and their individual isogenic controls generated via precision CRISPR/Cas9 genome editing.
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| Overall design |
RNA-seq study on human induced pluripotent stem cells differentiated in glutamatergic cortical neurons carrying A79V and L150P mutations in PSEN1, compared to gene-corrected controls.
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| Contributor(s) |
Corsi GI, Gadekar VP, Haukedal H, Doncheva NT, Anthon C, Ambardar S, Palakodeti D, Simonsen AH, Nielsen TT, Nielsen JE, Hyttel P, Freude K, Seemann SE, Gorodkin J |
| Citation(s) |
36572121, 36875643 |
| Submission date |
Aug 24, 2022 |
| Last update date |
Jun 14, 2023 |
| Contact name |
Christian Anthon |
| E-mail(s) |
anthon@rth.dk
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| Phone |
+4521510929
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| Organization name |
University of Copenhagen
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| Department |
Veterinary and Animal Sciences
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| Lab |
RTH
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| Street address |
Thorvaldsens Vej 57
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| City |
Frederiksberg C |
| ZIP/Postal code |
1871 |
| Country |
Denmark |
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| Platforms (1) |
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| Samples (40)
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| GSM6506917 |
A79V, Neu, Week7, RNA, S1 (polyA RNA) |
| GSM6506918 |
A79V, Neu, Week7, RNA, S2 (polyA RNA) |
| GSM6506919 |
A79V, Neu, Week7, RNA, S3 (polyA RNA) |
| GSM6506920 |
A79V, Neu, Week7, RNA, S4 (polyA RNA) |
| GSM6506921 |
A79V, Neu, Week7, RNA, S5 (polyA RNA) |
| GSM6506922 |
A79V, GC, Neu, Week7, RNA, S1 (polyA RNA) |
| GSM6506923 |
A79V, GC, Neu, Week7, RNA, S2 (polyA RNA) |
| GSM6506924 |
A79V, GC, Neu, Week7, RNA, S3 (polyA RNA) |
| GSM6506925 |
A79V, GC, Neu, Week7, RNA, S4 (polyA RNA) |
| GSM6506926 |
A79V, GC, Neu, Week7, RNA, S5 (polyA RNA) |
| GSM6506927 |
L150P, Neu, Week7, RNA, S1 (polyA RNA) |
| GSM6506928 |
L150P, Neu, Week7, RNA, S2 (polyA RNA) |
| GSM6506929 |
L150P, Neu, Week7, RNA, S3 (polyA RNA) |
| GSM6506930 |
L150P, Neu, Week7, RNA, S4 (polyA RNA) |
| GSM6506931 |
L150P, Neu, Week7, RNA, S5 (polyA RNA) |
| GSM6506932 |
L150P, GC, Neu, Week7, RNA, S1 (polyA RNA) |
| GSM6506933 |
L150P, GC, Neu, Week7, RNA, S2 (polyA RNA) |
| GSM6506934 |
L150P, GC, Neu, Week7, RNA, S3 (polyA RNA) |
| GSM6506935 |
L150P, GC, Neu, Week7, RNA, S4 (polyA RNA) |
| GSM6506936 |
L150P, GC, Neu, Week7, RNA, S5 (polyA RNA) |
| GSM6506937 |
A79V, Neu, Week7, RNA, S1 (total RNA) |
| GSM6506938 |
A79V, Neu, Week7, RNA, S2 (total RNA) |
| GSM6506939 |
A79V, Neu, Week7, RNA, S3 (total RNA) |
| GSM6506940 |
A79V, Neu, Week7, RNA, S4 (total RNA) |
| GSM6506941 |
A79V, Neu, Week7, RNA, S5 (total RNA) |
| GSM6506942 |
A79V, GC, Neu, Week7, RNA, S1 (total RNA) |
| GSM6506943 |
A79V, GC, Neu, Week7, RNA, S2 (total RNA) |
| GSM6506944 |
A79V, GC, Neu, Week7, RNA, S3 (total RNA) |
| GSM6506945 |
A79V, GC, Neu, Week7, RNA, S4 (total RNA) |
| GSM6506946 |
A79V, GC, Neu, Week7, RNA, S5 (total RNA) |
| GSM6506947 |
L150P, Neu, Week7, RNA, S1 (total RNA) |
| GSM6506948 |
L150P, Neu, Week7, RNA, S2 (total RNA) |
| GSM6506949 |
L150P, Neu, Week7, RNA, S3 (total RNA) |
| GSM6506950 |
L150P, Neu, Week7, RNA, S4 (total RNA) |
| GSM6506951 |
L150P, Neu, Week7, RNA, S5 (total RNA) |
| GSM6506952 |
L150P, GC, Neu, Week7, RNA, S1 (total RNA) |
| GSM6506953 |
L150P, GC, Neu, Week7, RNA, S2 (total RNA) |
| GSM6506954 |
L150P, GC, Neu, Week7, RNA, S3 (total RNA) |
| GSM6506955 |
L150P, GC, Neu, Week7, RNA, S4 (total RNA) |
| GSM6506956 |
L150P, GC, Neu, Week7, RNA, S5 (total RNA) |
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| Relations |
| BioProject |
PRJNA873113 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE211993_gencode_fantomcat.v1.02.genes_transcripts_exons.gtf.gz |
33.5 Mb |
(ftp)(http) |
GTF |
| GSE211993_uniquely_mapping-raw_counts_polyA.tsv.gz |
7.0 Mb |
(ftp)(http) |
TSV |
| GSE211993_uniquely_mapping-raw_counts_total.tsv.gz |
7.1 Mb |
(ftp)(http) |
TSV |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data are available on Series record |
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