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| Status |
Public on Jun 16, 2010 |
| Title |
Effects of Ionizing Radiation on Self Renewal and Pluripotency of Human Embryonic Stem Cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Human embryonic stem cells (hESCs) present a novel platform for in vitro investigation of the early embryonic cellular response to ionizing radiation. Thus far, no study has analyzed the genome-wide transcriptional response to ionizing radiation in hESCs. In this study, we use Agilent microarrays to analyze the global gene expression changes in H9 hESCs after low (0.4 Gy), medium (2 Gy), and high (4 Gy) dose irradiation.
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| Overall design |
Undifferentiated H9 hESCs were cultured on Matrigel in feeder-free conditions, and exposed to ionizing radiation at the indicated dosage (or control) from a Cesium-137 irradiator. Total RNA was isolated 24 hours after irradiation in the same feeder-free culture conditions. Experiment was repeated three times for each group, yielding a total of 12 distinct samples.
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| Contributor(s) |
Wilson KD, Huang M |
| Citation(s) |
20530673 |
| Submission date |
Mar 18, 2010 |
| Last update date |
Jan 23, 2019 |
| Contact name |
Kitchener D. Wilson |
| E-mail(s) |
kitchwilson@stanford.edu
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| Organization name |
Stanford University
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| Street address |
S140 Grant Bldg
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| City |
Stanford |
| State/province |
CA |
| ZIP/Postal code |
94305 |
| Country |
USA |
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| Platforms (1) |
| GPL6480 |
Agilent-014850 Whole Human Genome Microarray 4x44K G4112F (Probe Name version) |
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| Samples (12)
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| GSM523876 |
Control (non-irradiated) hESCs, Replicate 1 |
| GSM523877 |
Control (non-irradiated) hESCs, Replicate 2 |
| GSM523878 |
Control (non-irradiated) hESCs, Replicate 3 |
| GSM523879 |
0.4 Gy irradiated hESCs, Replicate 1 |
| GSM523880 |
0.4 Gy irradiated hESCs, Replicate 2 |
| GSM523881 |
0.4 Gy irradiated hESCs, Replicate 3 |
| GSM523882 |
2 Gy irradiated hESCs, Replicate 1 |
| GSM523883 |
2 Gy irradiated hESCs, Replicate 2 |
| GSM523884 |
2 Gy irradiated hESCs, Replicate 3 |
| GSM523885 |
4 Gy irradiated hESCs, Replicate 1 |
| GSM523886 |
4 Gy irradiated hESCs, Replicate 2 |
| GSM523887 |
4 Gy irradiated hESCs, Replicate 3 |
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| Relations |
| BioProject |
PRJNA124437 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE20951_RAW.tar |
186.4 Mb |
(http)(custom) |
TAR (of TXT) |
| Processed data included within Sample table |
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