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| Status |
Public on Feb 06, 2023 |
| Title |
Recycling of H2A-H2B provides short-term memory of chromatin states [ChIP-Seq] |
| Organisms |
Homo sapiens; Mus musculus |
| Experiment type |
Genome binding/occupancy profiling by high throughput sequencing
|
| Summary |
Chromatin landscapes are disrupted during DNA replication and must be restored faithfully to maintain genome regulation and cell identity. The H3-H4 modification landscape is restored by parental histone recycling and post-replication modification of new histone H3-H4. How DNA replication impact on histone H2A-H2B is unknown. Here, we track H2A-H2B modifications and H2A.Z during DNA replication and across the cell cycle using quantitative genomics. We show that H2AK119ub, H2BK120ub, and H2A.Z are recycled quantitatively and accurately during DNA replication. H2A-H2B are recycled symmetrically to daughter strands largely independent of known H3-H4 recycling pathways. Post-replication, H2A-H2B modifications are rapidly restored, and the rapid wave of H2AK119ub supports accurate restoration of H3K27me3. This work reveals epigenetic transmission of H2A-H2B modification during DNA replication and identifies H3-H4 and H2A-H2B crosstalk in epigenome propagation. We propose that rapid short-term memory of recycled H2A-H2B modifications facilitates reestablishment of slow, long-term chromatin state memory.
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| Overall design |
ChIP-Seq measuring chromatin occupancy for H3K27me3, H2AK119ub, H2A.Z, H2BK120ub, pan-H2A, pan-H3, JARID2, RING1B in wildtype mouse embryonic stem cells (WT cells) or Ring1B-mAID BAP1dTAG Ring1AKO or human HCT116 cells with corresponding Inputs in replicates.
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| Contributor(s) |
Flury V, Reverón-Gómez N, Alcaraz N, Stewart-Morgan K, Wenger A, Klose RJ, Groth A |
| Citation(s) |
36750094 |
| Submission date |
May 27, 2022 |
| Last update date |
May 08, 2023 |
| Contact name |
Anja Groth |
| E-mail(s) |
anja.groth@cpr.ku.dk
|
| Organization name |
Novo Nordisk Foundation Center for Protein Research
|
| Street address |
Blegdamsvej 3B
|
| City |
Copenhagen |
| ZIP/Postal code |
2200 |
| Country |
Denmark |
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| Platforms (3) |
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| Samples (54)
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| This SubSeries is part of SuperSeries: |
| GSE204988 |
Recycling of H2A-H2B provides short-term memory of chromatin states |
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| Relations |
| BioProject |
PRJNA842931 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE204974_H2AZ_replicated_final_noblacklist_n19677.bed.gz |
167.6 Kb |
(ftp)(http) |
BED |
| GSE204974_H2Aub_replicated_final_noblacklist_4703.bed.gz |
42.3 Kb |
(ftp)(http) |
BED |
| GSE204974_H2Bub_replicated_final_noblacklist_n21676.bed.gz |
185.2 Kb |
(ftp)(http) |
BED |
| GSE204974_H3K27me3_replicated_final_noblacklist_n11502.bed.gz |
99.4 Kb |
(ftp)(http) |
BED |
| GSE204974_HCT116_H2AZ_peaks_n64048.bed.gz |
527.9 Kb |
(ftp)(http) |
BED |
| GSE204974_HCT116_H2Bub_peaks_n93123.bed.gz |
729.3 Kb |
(ftp)(http) |
BED |
| GSE204974_RAW.tar |
4.2 Gb |
(http)(custom) |
TAR (of BW) |
| GSE204974_mES_JARID2_peaks_n6874.bed.gz |
61.4 Kb |
(ftp)(http) |
BED |
SRA Run Selector |
| Raw data are available in SRA |
| Processed data provided as supplementary file |
| Processed data are available on Series record |
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