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Series GSE2042

Query DataSets for GSE2042

Status

Public on Dec 21, 2005

Title

Apoptosis and differentiation commitment:novel insights revealed by gene profiling studies in mouse Embryonic Stem cells

Organism

Mus musculus

Experiment type

Expression profiling by array

Summary

Mouse embryonic stem (ES) cells remain pluripotent in vitro when grown in presence of Leukaemia Inhibitory Factor (LIF). LIF starvation leads to apoptosis of some of the ES-derived differentiated cells, together with p38a MAP kinase activation. Apoptosis, but not morphological cell differentiation, is blocked by a p38 inhibitor, PD 169316. To further understand the mechanism of action of this compound, we have identified its specific targets by microarray studies. We report on the global expression profiles of genes expressed at three days upon LIF withdrawal (d3) compared to pluripotent cells and of genes whose expression is modulated at d3 under anti-apoptotic conditions. We showed that at d3 without LIF cells express, earlier than anticipated, specialized cell markers and that when the apoptotic process was impaired, expression of differentiation markers was altered. In addition, functional tests revealed properties of anti-apoptotic proteins not to alter cell pluripotency and a novel role for metallothionein 1 gene which prevents apoptosis of early differentiated cells.

Keywords: mouse ES cells; apoptosis; differentiation; p38; PD169316;
Ca++ binding proteins; Metallothionein; stress-response
Keywords: ordered

Contributor(s)

Duval D, Trouillas M, Thibault C, Dembele D, Diemunsch F, Reinhardt B, Mertz A, Dierich A, Boeuf H

Citation(s)

16311515, 18437159

Submission date

Dec 06, 2004

Last update date

Feb 18, 2018

Contact name

Helene BOEUF

E-mail(s)

helene.boeuf@u-bordeaux2.fr