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| Status |
Public on Feb 11, 2010 |
| Title |
Silencing of C2TA reveals the autonomous role of medullary thymic epithelial cells in central CD4 T cell tolerance |
| Organism |
Mus musculus |
| Experiment type |
Expression profiling by array
|
| Summary |
[original title] Tissue-specific silencing of C2TA reveals the autonomous role of medullary thymic epithelial cells in central CD4 T cell tolerance.
Medullary thymic epithelial cells (mTECs) serve an essential function in central tolerance through expressing peripheral tissue-antigens. Because these antigens may be transferred to and presented by Dendritic Cells, it is unclear whether, besides being an ‘antigen reservoir’, mTECs also fulfill a critical antigen presenting cellfunction. We found that reducing MHC class II-levels on mTECs through transgenic expression of a C2TA-specific ‘designer miRNA’ resulted in an enlarged polyclonal CD4 single-positive compartment. Less CD4+ thymocytes specific for model-antigens expressed in mTECs were deleted, whereas more antigen-specific Foxp3+ regulatory T cells (Treg) emerged. Our data suggest a substantial autonomous contribution of mTECs to both dominant and recessive mechanisms of CD4 T cell tolerance and support an avidity model of Treg development versus deletion.
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| Overall design |
Three conditions: Wild-type, C2TA knockdown, and C2TA knock-out. For each condition three replicates and in total 9 arrays.
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| Contributor(s) |
Hinterberger M, Aichinger M, Voehringer D, Prazeres da Costa O, Hoffmann R, Klein L |
| Citation(s) |
20431619 |
| Submission date |
Feb 10, 2010 |
| Last update date |
Mar 04, 2019 |
| Contact name |
Maria Hinterberger |
| E-mail(s) |
maria.hinterberger@med.uni-muenchen.de
|
| Organization name |
LMU
|
| Department |
Institut für Immunologie
|
| Lab |
AG Klein
|
| Street address |
Goethestr. 31
|
| City |
Munich |
| ZIP/Postal code |
80336 |
| Country |
Germany |
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| Platforms (1) |
| GPL6246 |
[MoGene-1_0-st] Affymetrix Mouse Gene 1.0 ST Array [transcript (gene) version] |
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| Samples (9)
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| Relations |
| BioProject |
PRJNA125365 |
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