|
| Status |
Public on Jan 06, 2012 |
| Title |
STK38 is a Key Regulator of MYC Transcriptional Activity in Human B lymphoma cells |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
Post-translational regulation of the MYC Transcription Factor (TF), including its phosphorylation and ubiquitination, plays an important role in determining cell proliferation and apoptosis and has been implicated in tumorigenesis. Using a computational systems biology approach, followed by biochemical and functional validation, we have characterized the role of the STK38 kinase, an NDR family serine-threonine kinase, as a key modulator of MYC transcriptional activity in human B cells, affecting MYC protein stability in a signal-dependent fashion. Specifically, we show that in human B lymphoma ST486 cells STK38 is a key mediator of BCR pathway signaling, affecting MYC protein turnover and its phosphorylation at Ser62 in kinase-activity-dependent manner. STK38 inactivation abrogates apoptosis following BCR activation while its silencing mediates MYC protein degradation via canonical proteolytic pathways. This suggests that STK38 could provide an effective therapeutic target in MYC-dependent malignancies.
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| |
|
| Overall design |
ST486 human Burkitt's lymphoma cells were transduced with STK38 shRNA lentiviral vectors.
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| |
|
| Citation(s) |
23178486 |
| Submission date |
Feb 08, 2010 |
| Last update date |
Dec 13, 2018 |
| Contact name |
Mariano Javier Alvarez |
| Organization name |
Columbia University
|
| Department |
Systems Biology
|
| Lab |
Systems Biology
|
| Street address |
1130 St. Nicholas ave.
|
| City |
New York |
| State/province |
NY |
| ZIP/Postal code |
10032 |
| Country |
USA |
| |
|
| Platforms (1) |
| GPL8300 |
[HG_U95Av2] Affymetrix Human Genome U95 Version 2 Array |
|
| Samples (10)
|
|
| Relations |
| BioProject |
PRJNA124165 |
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