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| Status |
Public on Jan 07, 2010 |
| Title |
Expression Data From HCMV-Infected Human Monocytes 48 Hours Post-Infection: Role of PI(3)K |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
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| Summary |
Human cytomegalovirus (HCMV) induces pro-inflammatory monocytes following infection and we have evidence that phosphatidylinositol 3-kinase [PI(3)K] is a key mediator in this activation. To begin to address how this signalling pathway is responsible for the functional changes in infected monocytes, we examined the role this pathway played in the transcriptome of infected monocytes. Global transcriptional profiling using cDNA microarrays revealed a significant number of genes were regulated in a PI(3)K-dependent manner, identifying this pathway as a key cellular control point in the conversion of monocytes to an activated pro-inflammatory state following HCMV infection.
Keywords: Disease state
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| Overall design |
To begin to globally define how PI(3)K is involved in the HCMV-induced changes in monocyte function, we performed a transcriptome analysis in the presence of an inhibitor to the PI(3)K signalling pathway. Specifically, a cDNA microarray containing 12,626 unique probe sets was utilized to assess the modulation of the monocyte transcriptome at 48 hours post-infection in the presence of the pharmacological agent LY294002 (LY), a PI(3)K inhibitor. A total of 2 replicates from HCMV-infected monocytes, 2 replicates from LY-pretreated infected monocytes, and 2 replicates from mock-infected monocytes were analyzed in this study.
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| Contributor(s) |
Bivins-Smith ER, Smith MS, Yurochko AD |
| Citation(s) |
20173022 |
| Submission date |
Jan 06, 2010 |
| Last update date |
Dec 13, 2018 |
| Contact name |
Andrew D. Yurochko |
| E-mail(s) |
ayuroc@lsuhsc.edu
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| Phone |
318-675-8332
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| Fax |
318-675-5764
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| Organization name |
LSUHSC-S
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| Department |
Microbiology & Immunology
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| Street address |
1501 Kings Highway
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| City |
Shreveport |
| State/province |
LA |
| ZIP/Postal code |
71130-3932 |
| Country |
USA |
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| Platforms (1) |
| GPL8300 |
[HG_U95Av2] Affymetrix Human Genome U95 Version 2 Array |
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| Samples (6)
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| GSM493878 |
Mock-infected monocyte, 48 hr post-infection, biological rep1 |
| GSM493879 |
Mock-infected monocyte, 48 hr post-infection, biological rep2 |
| GSM493880 |
HCMV-infected monocyte, 48 hr post-infection, biological rep1 |
| GSM493881 |
HCMV-infected monocyte, 48 hr post-infection, biological rep2 |
| GSM493882 |
HCMV-infected/LY-pretreated monocyte, 48 hr post-infection, biological rep1 |
| GSM493883 |
HCMV-infected/LY-pretreated monocyte, 48 hr post-infection, biological rep2 |
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| Relations |
| BioProject |
PRJNA121855 |
| Supplementary file |
Size |
Download |
File type/resource |
| GSE19772_RAW.tar |
40.0 Mb |
(http)(custom) |
TAR (of CEL, CHP) |
| Processed data included within Sample table |
| Processed data provided as supplementary file |
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