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| Status |
Public on Feb 06, 2023 |
| Title |
A global transcriptome analysis of human dermal fibroblasts upon knockdown of the circular RNAs circGLIS3 (circBase ID: hsa_circ_0002874) |
| Organism |
Homo sapiens |
| Experiment type |
Expression profiling by array
|
| Summary |
In an RNAseq analysis, we have identified circGLIS3 with high levels acute wound dermis compared to skin
The biological function of circGLIS3 in human dermal fibroblasts during wound repair has not been studied.
To study the genes regulated by circGLIS3, we transfected siRNA targeting the diagnostic junctions of circGLIS3 into human dermal fibroblasts to knockdown circGLIS3 expression.
We performed a global transcriptome analysis of keratinocytes upon circRNA knockdown using Affymetrix arrays.
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| Overall design |
Gene expression profiling of keratinocytes transfected with either siRNA control (n = 3) or si-circGLIS3 (n = 3) for 24 hours was carried out by using human Clariom™ S assay (ThermoFisher Scientific) at the Bioinformatics and Expression Analysis (BEA) core facility at Karolinska Institutet.
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| Contributor(s) |
Landén NX, Toma MA |
| Citation missing |
Has this study been published? Please login to update or notify GEO. |
| Submission date |
Feb 07, 2022 |
| Last update date |
Feb 08, 2023 |
| Contact name |
Maria-Alexandra Toma |
| E-mail(s) |
maria.toma@ki.se
|
| Phone |
+46720056044
|
| Organization name |
Karolinska Institute
|
| Department |
Medicine
|
| Lab |
Ning Xu Landén
|
| Street address |
Karolinska vägen 6
|
| City |
Stockholm |
| ZIP/Postal code |
171 76 |
| Country |
Sweden |
| |
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| Platforms (1) |
| GPL23159 |
[Clariom_S_Human] Affymetrix Clariom S Assay, Human (Includes Pico Assay) |
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| Samples (6)
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| GSM5866616 |
si-ctrl transfected fibroblasts, biological replicate 1 |
| GSM5866617 |
si-ctrl transfected fibroblasts, biological replicate 2 |
| GSM5866618 |
si-ctrl transfected fibroblasts, biological replicate 3 |
| GSM5866619 |
si-cGLIS3 transfected fibroblasts, biological replicate 1 |
| GSM5866620 |
si-cGLIS3 transfected fibroblasts, biological replicate 2 |
| GSM5866621 |
si-cGLIS3 transfected fibroblasts, biological replicate 3 |
|
| Relations |
| BioProject |
PRJNA804103 |
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